Detailed Plasmid Information

Explanation of Terms

Gene:
Gene Symbol: 
Gene Name: 
Original Clone ID:
976.1.23_GO.34568
Keyword:
None
Species:
Mus musculus
Type:
cDNA
Vector Name:
pVP16
Format: 
CLOSED
Source:
Center for Eukaryotic Structural Genomics
Description:
None
Comments:
His8-MBP fusion prtotein with TEV protease cleavage site is produced; after cleavage the protein obtained will be the translated CDS with the addition of SER at the N-term
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
PSI
Center for Eukaryotic Structural Genomics
MmCD00089227
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AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 1296nts
Open reading frame: 28 to 1323

ACAAGTTTGTACAAAAAAGCAGGCTCCGAAAACCTGTACTTCCAGTCCGCTCCAGAGGAG
GACGCCGGAGGGGAGGTCCTAGGGGGCAGTTTCTGGGAGGCTGGCAACTACAGACGGACG
GTGCAGAGGGTTGAGGATGGGCACAGGCTGTGTGGGGACCTGGTTAGCTGCTTCCAAGAA
CGTGCCCGCATTGAGAAGGCCTACGCCCAGCAGCTGGCTGACTGGGCCCGCAAGTGGAGG
GGTGCTGTGGAGAAGGGCCCACAGTACGGCACCCTGGAGAAGGCCTGGCATGCCTTCTTC
ACTGCGGCTGAGAGGCTGAGCGAGCTGCACTTGGAGGTGAGGGAGAAGTTGCACGGTCCA
GACAGCGAGCGAGTGCGGACCTGGCAGCGAGGGGCTTTCCACCGGCCAGTGCTGGGGGGC
TTTCGGGAAAGCCGGGCTGCAGAGGATGGTTTCCGTAAAGCTCAGAAGCCCTGGCTAAAG
AGGCTGAAGGAGGTTGAGGCTTCTAAGAAGAGCTACCACACAGCCCGCAAGGATGAGAAG
ACAGCCCAGACCCGGGAGAGCCACGCGAAGGCAGACAGCTCCATGTCCCAGGAGCAGCTT
CGAAAATTACAGGAGCGGGTGGGCCGCTGCACCAAGGAGGCAGAGAAGATGAAAACCCAG
TATGAGCAGACCCTGGCCGAGCTAAATCGCTACACCCCACGCTACATGGAGGACATGGAG
CAGGCTTTTGAGAGCTGCCAGGCTGCTGAGCGCCAGCGGCTTCTCTTCTTCAAGGATGTC
TTGCTCACCCTGCATCAACACCTCGACCTCTCCAGTAGCGACAAGTTCCATGAACTCCAT
CGAGACCTGCAGCAGAGCATTGAGGCTGCCAGTGATGAAGAGGATCTGCGCTGGTGGCGG
AGCACACACGGGCCTGGCATGGCCATGAACTGGCCACAGTTTGAGGAATGGTCGTTAGAC
ACACAGAGAGCCATCAGCCGCAAGGAGAAGGGTGGCCGGAGCCCAGATGAGGTTACTCTG
ACCAGCATTGTACCGACAAGAGATGGCACCGCACCCCCACCCCAGTCCCCATCGTCCCCG
GGTAGCGGGCAGGACGAGGACTGGTCAGATGAGGAGAGTCCCCGGAAAGTTGCCACTGGG
GTGAGGGTACGGGCGCTTTATGACTATGCTGGCCAGGAGGCTGATGAGCTGAGCTTCCGA
GCAGGGGAAGAGTTGCTGAAGATGAGCGAGGAGGATGAGCAGGGCTGGTGCCAGGGCCAG
CTGCAGAGTGGCCGCATTGGTCTGTACCCTGCCAATTACGTAGAATGTGTGGGCGCCTGA
TAGGACCCAGCTTTCTTGTACAAAGTGGT

Protein sequence predicted based on nucleotide sequence

ENLYFQSAPEEDAGGEVLGGSFWEAGNYRRTVQRVEDGHRLCGDLVSCFQERARIEKAYA
QQLADWARKWRGAVEKGPQYGTLEKAWHAFFTAAERLSELHLEVREKLHGPDSERVRTWQ
RGAFHRPVLGGFRESRAAEDGFRKAQKPWLKRLKEVEASKKSYHTARKDEKTAQTRESHA
KADSSMSQEQLRKLQERVGRCTKEAEKMKTQYEQTLAELNRYTPRYMEDMEQAFESCQAA
ERQRLLFFKDVLLTLHQHLDLSSSDKFHELHRDLQQSIEAASDEEDLRWWRSTHGPGMAM
NWPQFEEWSLDTQRAISRKEKGGRSPDEVTLTSIVPTRDGTAPPPQSPSSPGSGQDEDWS
DEESPRKVATGVRVRALYDYAGQEADELSFRAGEELLKMSEEDEQGWCQGQLQSGRIGLY
PANYVECVGA**

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence 1
End on reference sequence 1323
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition: 100 ug/mL ampicillin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
ProteinExpressed: Protein_Confirmed
ProteinPurified: Protein_Purified
SolubleProtein: Protein_Soluble
Find experimental protocols in GO.34568
Recommended expression in:
Not Applicable