DNASU Plasmid | TM1839 (T. maritima ) in pMH2T7 (bacterial expression vector)

Explanation of Terms

Gene:

Gene Symbol: TM1839

Gene Name: maltose ABC transporter substrate-binding protein

Original Clone ID:

8000

Keyword:

None

Species:

Thermotoga maritima

Type:

cDNA

Vector Name:

pMH2T7

Format:

CLOSED

Source:

Joint Center for Structural Genomics

Description:

None

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

PSI
Joint Center for Structural Genomics

TmCD00089498

Price:

AVAILABLE

No restriction
Special MTA: PSI

Insert Sequence
Protein Sequence

Insert sequence: 1182nts

Open reading frame: 51 to 1232

AGATATACATACCCATGGGATCTGATAAAATTCATCATCATCATCATCACATGGTGAAGA
GACTGCTCGTTTTAATGCTTGTTGTGGTTTCTGCTCTGGTGTTATCACAAACAAAGCTCA
CCATCTGGTGTTCCGAAAAGCAGGTTGACATCCTCCAGAAACTCGGGGAAGAATTCAAGG
CAAAGTACGGAATCCCTGTTGAAGTTCAGTACGTCGATTTTGGAAGCATCAAATCCAAAT
TCCTGACGGCGGCTCCACAGGGACAGGGCGCAGACATCATTGTTGGAGCGCACGACTGGG
TAGGAGAACTCGCCGTCAACGGTTTGATCGAACCCATTCCCAACTTCTCTGATCTGAAGA
ATTTCTATGACACGGCTCTCAAAGCTTTCTCTTACGGTGGAAAACTCTACGGAGTCCCGT
ACGCCATGGAAGCGGTTGCTCTCATCTACAACAAGGACTACGTTGATTCTGTTCCTAAGA
CCATGGACGAGCTCATAGAAAAAGCAAAACAGATAGATGAGGAATACGGAGGAGAAGTCA
GAGGTTTCATCTACGATGTCGCCAACTTCTACTTCTCTGCGCCGTTCATTCTAGGTTACG
GAGGATACGTCTTCAAGGAAACACCTCAGGGACTCGACGTGACAGACATTGGACTCGCGA
ACGAAGGAGCAGTCAAAGGTGCGAAACTCATAAAGAGAATGATCGATGAAGGTGTTCTCA
CCCCTGGTGACAACTACGGAACGATGGATTCCATGTTCAAAGAAGGTCTCGCGGCTATGA
TCATCAACGGACTTTGGGCTATAAAATCCTACAAAGATGCAGGTATAAACTACGGAGTCG
CTCCTATTCCTGAGCTCGAGCCGGGTGTTCCTGCCAAACCGTTTGTTGGTGTTCAGGGAT
TCATGATCAATGCCAAGTCTCCAAACAAAGTGATCGCTATGGAATTTCTCACGAACTTCA
TTGCGAGAAAAGAAACCATGTACAAGATATACCTCGCAGATCCAAGGCTTCCTGCGAGAA
AAGATGTTCTTGAACTCGTCAAAGACAATCCCGATGTTGTTGCGTTCACCCAGAGTGCTT
CCATGGGAACACCGATGCCAAACGTGCCGGAAATGGCTCCTGTCTGGTCTGCCATGGGAG
ACGCTCTCAGCATCATTATCAACGGACAGGCCAGTGTCGAAGATGCTCTCAAAGAGGCTG
TGGAAAAAATCAAGGCACAGATAGAAAAATAATTAATTAACTTTAAGAAGGAGATATACA
TACCCGGGTAAGTTTAAACGGT

Protein sequence predicted based on nucleotide sequence

MVKRLLVLMLVVVSALVLSQTKLTIWCSEKQVDILQKLGEEFKAKYGIPVEVQYVDFGSI
KSKFLTAAPQGQGADIIVGAHDWVGELAVNGLIEPIPNFSDLKNFYDTALKAFSYGGKLY
GVPYAMEAVALIYNKDYVDSVPKTMDELIEKAKQIDEEYGGEVRGFIYDVANFYFSAPFI
LGYGGYVFKETPQGLDVTDIGLANEGAVKGAKLIKRMIDEGVLTPGDNYGTMDSMFKEGL
AAMIINGLWAIKSYKDAGINYGVAPIPELEPGVPAKPFVGVQGFMINAKSPNKVIAMEFL
TNFIARKETMYKIYLADPRLPARKDVLELVKDNPDVVAFTQSASMGTPMPNVPEMAPVWS
AMGDALSIIINGQASVEDALKEAVEKIKAQIEK*

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Sequence Verification

Reference Sequence Annotations:

Start on reference sequence 1

End on reference sequence 1232

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DL41

Antibiotic Selection:

Host Type: bacterial Marker: ampicillin

Bacterial Selection Condition: 100 ug/mL ampicillin

Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.

Comments: Commonly used conditions for ampicillin resistant plasmid clones.

Protein Expression Results:

ProteinExpressed: Not_Applicable

ProteinPurified: Protein_Purified

SolubleProtein: Tested_Not_Soluble

Find experimental protocols in 283691

Recommended expression in:

Not Applicable

Vector Name:

pMH2T7

Synonyms:

pMH-2-T7

Sequencing Primer:

Forward: pBAD forward

Reverse: alt_bad_rev

Description:

Bacterial (E. coli) expression vector, arabinose or T7 induced expression, adds N-terminal MGSDKIHHHHHH tag; amp resistance; restriction enzyme (Pml I/Pac I) or PIPE cloning.

Comments:

None

Size (bp):

4084

Parent Vector:

None

Properties:

PIPE cloning, bacterial expression, in vitro transcription, inducible expression, with tag/fusion/marker

Author Name:

Joint Center for Structural Genomics

Publications:

None

Vector Map:

Vector Sequence:

Sequence:

AAGAAACCAATTGTCCATATTGCATCAGACATTGCCGTCACTGCGTCTTTTACTGGCTCTTCTCGCTAACCAAACCGGTAACCCCGCTTATTAAAAGCATTCTGTAACAAAGCGGGACCAAAGCCATGACAAAAACGCGTAACAAAAGTGTCTATAATCACGGCAGAAAAGTCCACATTGATTATTTGCACGGCGTCACACTTTGCTATGCCATAGCATTTTTATCCATAAGATTAGCGGATCCTACCTGACGCTTTTTATCGCAACTCTCTACTGTTTCTCCATACCCGTAATACGACTCACTATAGGGTTTTGTTTAACTTTAAGAAGGAGATATACATACCCATGGGATCTGATAAAATTCATCATCATCATCATCACGTGTAATTAATTAACTTTAAGAAGGAGATATACATACCCGGGTAAGTTTAAACGGTCTCCAGCTTGGCTGTTTTGGCGGATGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTTTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTGTTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACTTCATTTTTAATTTAAAAGGATCTAGGTGAAGATCCTTTTTGATAATCTCATGACCAAAATCCCTTAACGTGAGTTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTCACACCGCATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGTATACACTCCGCTATCGCTACGTGACTGGGTCATGGCTGCGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGGCAGCAGATCAATTCGCGCGCGAAGGCGAAGCGGCATGCATAATGTGCCTGTCAAATGGACGAAGCAGGGATTCTGCAAACCCTATGCTACTCCGTCAAGCCGTCAATTGTCTGATTCGTTACCAATTATGACAACTTGACGGCTACATCATTCACTTTTTCTTCACAACCGGCACGGAACTCGCTCGGGCTGGCCCCGGTGCATTTTTTAAATACCCGCGAGAAATAGAGTTGATCGTCAAAACCAACATTGCGACCGACGGTGGCGATAGGCATCCGGGTGGTGCTCAAAAGCAGCTTCGCCTGGCTGATACGTTGGTCCTCGCGCCAGCTTAAGACGCTAATCCCTAACTGCTGGCGGAAAAGATGTGACAGACGCGACGGCGACAAGCAAACATGCTGTGCGACGCTGGCGATATCAAAATTGCTGTCTGCCAGGTGATCGCTGATGTACTGACAAGCCTCGCGTACCCGATTATCCATCGGTGGATGGAGCGACTCGTTAATCGCTTCCATGCGCCGCAGTAACAATTGCTCAAGCAGATTTATCGCCAGCAGCTCCGAATAGCGCCCTTCCCCTTGCCCGGCGTTAATGATTTGCCCAAACAGGTCGCTGAAATGCGGCTGGTGCGCTTCATCCGGGCGAAAGAACCCCGTATTGGCAAATATTGACGGCCAGTTAAGCCATTCATGCCAGTAGGCGCGCGGACGAAAGTAAACCCACTGGTGATACCATTCGCGAGCCTCCGGATGACGACCGTAGTGATGAATCTCTCCTGGCGGGAACAGCAAAATATCACCCGGTCGGCAAACAAATTCTCGTCCCTGATTTTTCACCACCCCCTGACCGCGAATGGTGAGATTGAGAATATAACCTTTCATTCCCAGCGGTCGGTCGATAAAAAAATCGAGATAACCGTTGGCCTCAATCGGCGTTAAACCCGCCACCAGATGGGCATTAAACGAGTATCCCGGCAGCAGGGGATCATTTTGCGCTTCAGCCATACTTTTCATACTCCCGCCATTCAGAG

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ori

ColEI origin of replication

1921

2603

promoter

T7 promoter

290

310

promoter

araC

araC promoter

256

285

selectable marker

AmpR

ampicillin resistance gene

1164

1823

tag

His

N-terminal 6x His tag

367

381

trxn regulatory element

arabinose O1 operator

161

182

trxn regulatory element

arabinose O2 operator

Gene

Gene Symbol :

TM1839

GENEID :

897815

Locus Tag :

TM1839

GI :

15644582

GenBank Accession :

NP_229635.1

Target GenBank:

NP_229635

Protein

SMART domain :

Proteasome_A_N : Proteasome subunit A N-terminal signature Add an annotation

Structural Annotation Wiki :

TOPSAN

Swissprot ID :

Q9S5Y1

Clone

Cloning Information :

276824

HIP Master Clone ID :

206310

Original Clone ID :

8000

Experimental

TargetTrack - Experimental data :

283691

Organism

Species Specific ID:

897815