Gene:
Gene Symbol:
TM1161
Gene Name:
Mg2+ transporter MgtE
Original Clone ID:
24527
Keyword:
None
Species:
Thermotoga maritima
Type:
cDNA
Vector Name:
pMH4TEV
Format:
CLOSED
Source:
Joint Center for Structural Genomics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
PSI
Joint Center for Structural Genomics
Joint Center for Structural Genomics
TmCD00089946
Price:
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AVAILABLE
No restriction
Special MTA: PSI
Special MTA: PSI
Insert sequence: 1341nts
Open reading frame: 51 to 1391
CTGATAAAATTCATCATCATCATCATCACGAAAACCTGTACTTCCAGGGCATGAAGATCA
AGGTAGAGATCGATCTTCAGGAATTGATCGAAAAAGGAGACTTCAAGACGCTCAAAAGGG
TTCTCGAACAGCAGGATCCCGCCGATGTGAAAGAGATGATCGAAAAGCTCCCGCCCGACC
TGAAAGTGGTTGTTTTCAGGCTCCTTCCCAAAGATAAAGCTGCTGAAGTGTTCAGTGAAC
TCGAACCCGACGATCAGCTGGAACTCATAAAGCTTTTCAGAGAGGAACGCCTGAAAGAGA
TCTTCGAATCGATGGATCCGGACGACAGAGTGGAACTACTCGAAGAGATGCCCGCAAACG
TGGTGAAAAAACTCCTTTCGTACCTTCCTCCACAGGAAAGGGAGGAACTTCTTTACATCC
TGAATTATCCTGAAGATTCTGCGGCAAGACTCGCCACTACGGAATACGTAGAGCTTTTCG
AGGATATGACCGTCAGAGAAGCGCTGGAAAAGGTGAGAAAAGAAGGAAAGAAAAAAGAGA
ATATCTACTCTCTCATGGTGATAGATAGAACGAGAAAGCTCAAGGGAACCGTTGAACTCA
GAGACCTGATAGTTGCCGATCCCGATCAGAAGGTATCAGAAATCATGAACAAAGATCCTG
TTTTTGTTCACGCCATCGATGACCAGGAACTCGCGGCAGAACTCATGAAGAAGTACGACC
TGATCATACTACCGGTGGTTGACAGCGAAGAGAGATTGATAGGTGTGATCACTTTCGACG
ATCTCGTTGACGTCATCGAAGAAGAGGCAACGGAAGACATACAGAAGATGGCCTCAATGA
GTGCTACGTACACGTCTTATTTTCACACTCCCGCGTGGAAACTCATCCTCAAAAGATCTC
CATGGCTTGTGGTACTCCTTCTCCTCGAGAGCGTCAACGGGAACATCATATCAAGCTACG
AGAAATTTCTCGCCTCAATACCGATCATAGCAGCTTTCATACCGACAATGATAGGTTCTG
CGGGTAACACAGGAGCGCAGATATCTGCACTCATGATAAGAGGATTCACACTGAACGAGA
TAAGTTTGAAAGACTGGTGGAAGGTTCTGTTGAGGGAATCTCTGATAGGTTCCACCTTGG
GTTTGATTCTTGCGGGTGTTCTCTACCTGAGGGCTTTTCTGATCTCTTCTGATCCCACAC
TGAATTTCGCCGTAGCAACGGCCTTGCTCGTGTTGATTCTTTACGCGAACATCATGGGAG
CGCTGCTTCCGTTCATCGCCCGTATTTTCAAAATCGACCCGGCCTTCATGGCAGGGCCTC
TTCTCACCACGATCGTCGACGTGACAGGCATCATGATATATTTCTACGTGGTGCACAGCT
TCTTGAGTTAACGCGACTTAATTAACTCTAGATAACTGAGAAGGAGATATACATACCCGG
G
Protein sequence predicted based on nucleotide sequence
MKIKVEIDLQELIEKGDFKTLKRVLEQQDPADVKEMIEKLPPDLKVVVFRLLPKDKAAEV FSELEPDDQLELIKLFREERLKEIFESMDPDDRVELLEEMPANVVKKLLSYLPPQEREEL LYILNYPEDSAARLATTEYVELFEDMTVREALEKVRKEGKKKENIYSLMVIDRTRKLKGT VELRDLIVADPDQKVSEIMNKDPVFVHAIDDQELAAELMKKYDLIILPVVDSEERLIGVI TFDDLVDVIEEEATEDIQKMASMSATYTSYFHTPAWKLILKRSPWLVVLLLLESVNGNII SSYEKFLASIPIIAAFIPTMIGSAGNTGAQISALMIRGFTLNEISLKDWWKVLLRESLIG STLGLILAGVLYLRAFLISSDPTLNFAVATALLVLILYANIMGALLPFIARIFKIDPAFM AGPLLTTIVDVTGIMIYFYVVHSFLS*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence
1
End on reference sequence
1391
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
ProteinExpressed: Not_Applicable
ProteinPurified: Protein_Purified
SolubleProtein: Tested_Not_Soluble
Find experimental protocols in 283027
Recommended expression in:
Not Applicable
Vector Name:
pMH4TEV
Synonyms:
pMH-4-TEV
Sequencing Primer:
Forward:
pBAD forward
Reverse:
alt_bad_rev
Description:
Bacterial expression vector, arabinose or T7 induced expression, adds N-terminal MGSDKIHHHHHHENLYFQG tag; amp resistance; PIPE cloning.
Comments:
None
Size (bp):
5510
Parent Vector:
None
Properties:
PIPE cloning, bacterial expression, in vitro transcription, inducible expression, with tag/fusion/marker
Author Name:
Joint Center for Structural Genomics
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ori
ColE1-type bacterial origin of replication
3352
4034
inducible trxn activator protein gene
araC
araC cds
5484
4606
negative selection marker
ccdB
ccdB negative selection cds (may be fone in with-insert form)
1597
1806
promoter
T7
T7 promoter
290
310
protease cleavage site
TEV
TEV protease cleavage site
382
402
selectable marker
CmR
chloramphenicol resistance gene (CmR)
505
1161
selectable marker
AmpR
ampicillin resistance gene
2595
3254
tag
His
N-terminal 6xHis tag
364
381
trxn regulatory element
arabinose O1 operator
arabinose O1 operator
161
182
trxn regulatory element
arabinose O2 operator
arabinose O2 operator
5
23
trxn termination sequence
rrnB
rrnB T2 transcriptional terminator
2259
2285
