ASK10 (S. cerevisiae ) in pDONR201 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol: 
Gene Name: 
Original Clone ID:
FLH003012.01X
Keyword:
None
Species:
Saccharomyces cerevisiae
Type:
cDNA
Vector Name:
pDONR201
Format: 
CLOSED
Source:
HIP
Description:
None
Comments:
None
Mutation / Discrepancy:
No / Yes
Publications:
None
Authors:
HIP
ScCD00093515
Price: 
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 3441nts
Open reading frame: 1 to 3441

ATGTCTGATTACTTTAGTTCCAGACCTTCGCAAACGCTCACGCCGATGGGCAATAAGCCA
TCTGGCGGTGGTGGCGGCGATGACGCCTCCTCCATCCACTCGAAGAGCTCGCAGTACTTG
ATGGACATCTTACCTGATTCGATGACCTTGAACGAAAGTGTTTCCTCCATAGTGGCAAAC
AATCAGGCAAAGGAGTTTATTTTGCCTGAAACAGATGAGAGATCTCCCTACTTCATAAAC
GTCCCTATACCAAAGGCTCAGCCAACCTCTACTACGGAAACGAAGAAGCCCCTTGCAGGT
GATGAAGCTATCGATGGGCAGTTTGTTAAGGAGTATCCCACCGACATCCTTGTAGACAGG
TTTTATAAATGGAAAAAAATTCTGAAAGGGTTAGTGATTTACTTGAGGGAAGTCGCGTAT
GCGCAAGAACAGTTCGCACGGATCAATTACCAACTGAAAGGATCTGTGAAATTTCCATTT
TTGACTGATATTGATGAAACCACAAATACCATTACGGATCCATTCACAACGGCTCCTAGA
GGCCCTAAGAAAGCGCAGCCTGCGCAGAAGAAAGTTGGCCTGACTGACAGCGAACAATTT
CAGATGCAAATGCAACAGGAACAGCAGGAAAATGCAGTGCAGGCTCCCACAGATGAAAGT
AAAATGAGTTTGGCGCCTCATGAATACAAGCCTGTCCAGACCACAGAGTCGGACAATACA
TCTGCCGCATCCGGTTTTGTTAAGTTCGGTTCAGGCTCGATCCAAGACATCCAGGTCATT
TTGAAGAAGTACCATCTTTCGTTGGCTAACCAGCAATTTAAGATCTCTAAAGAGATTACG
TCCACCGTCATTCCTAAATTGGAGGAATTGAGAAAAGATTTAAGATATAAAATTACGGAA
ATTAAGGACCTTCATGGTGATTTCAAAACAAACATCGGAGCACATATTCAGCTAACAAGT
CAACTATTGAAAAAATACATCGCAGCTGTAAAATTCATGAACGCGCATGGTATTGGCAAC
GATAGAGCATCTCCTACTAATAAGAAGCCACATAAATTGGATCCAAAACATGATCCATAT
CTTTTGAAACTGCAACTAGACTTACAACTCAAACGTCAAGTTGCCGAAGAAACTTACTTG
CAAGAAGCGTTCATCAATTTACAGAGTTCAGGTTTGCAATTAGAAAAAATTATATACACC
AAAATCCAGCATGCTTTGTTGCGTTATTCTGCTTTAATTGACTCCGAAGCTCGTCTAATG
ATCAAAAATATGTGCCAAGAACTACAACATGATATAATATCAAAGCCTCCTGCATTTGAA
TGGGATAATTTTGTCACACAGCATCCGTCCTGTCTACTCAATTGGAAATCCAACGATCCT
ATACCACCACCAAGAAAAGTTTCCGACGTTATTTATCCCCACATGAAATCCCCACTGGCC
AAGTGTATTAAGGCAGGTTATTTTTTGAAGAAATCGGAATTGTTGCCCACTTACCACCAA
GGATATTTTGTTCTAACATCAAACTATATTCATGAATTCCAGAGTAGCGATTTTTATAAC
CTATCTTCTTCTACCCCTAACTCCACGAAGTCTTCAGCTTATTCTTCTTCCGTTTCCATT
GCAGACACTTATGCCAATGCTAATAATGCCAAAGCTAACAATCATCATCGCCAGGCTAGT
GATGTACATAACAGCAGTACGACAACTGGTGGTACAGCTGGTGCAAATGGTATTCGCGGT
ATTCGCAAAAAGAGCTATTTGGCACCGATTATGAGTATTCCGCTAAATGATTGTACCTTA
AAGGATGCATCTTCCACCAAATTTGTTCTTGTAGGTAAACCCACTCTTAATGAGAACGCT
GATGTTAGAAAATCTAGCTCTAGCACTTATTTATCTGGCTCTTCACAAGCGTCTTTACCA
AAATATGGCCACGAGACTGCAAAAATATTTTCAAAAGCCCCATTCCACAAGTTTTTGAAG
GGAAGTAAGCCCAAAAATAAGAATACGAAATCAAGTGAACTGGACCAATTTTACGCTGCA
GCTCAAAAGGAATCGAACAATTATGTGACTTGGACCTTTAAAATAGTATCTCCAGAGCCT
TCTGAAGAGGAATTGAAACATTTTAAACGTTGGGTCCAAGATTTGAAAAATTTAACGAGC
TTCAATGACACTAAAGATAGAATTAAGTTTATCGAAGACCGTGTGATGAAATCACACCGA
TTCAAGGCGGGACATATGTCAAGAAACAGCGTGAACATAGGTTCCCATACACCTTGTTTA
ACAGATAGCACATTCACATTACAAGACGGTACTACGACGTCCGTCAACCTAAAGGGCAGG
GCGGAGAAACCTCAGTATATTCATATTCAGAACAATTCACTAGCAGATTTTGATGGAAAT
GGTTTTAGATCCAAGGTTAATACACCTGCTATTGACGATTATGGCAATTTGATTACGGTG
GAAAGAAGACCTGCACAATCTCCGCACCAATACTCTGACTACATGGCCACTTCAGGTAAC
ACAACTCCTTCTTATTCATCGGGCTCAGGACCTCAAAGCATGTATAATGGGTATAACCCG
GCTGTATCAATAACGAGTAATGGGATGATGCTCCAACAATCAACAGCAAATAATAATACT
AATCCAACAACTAATTTGCGCCACCAAAGAAATATTTCACAAACAAGTTCCCTACCTGGA
TTTTCATACACATCCCTATCCCTACCAGTGAATTCTCCAGGTAGCTCTAATTCAGAATCC
AGCTCAGGCGGTTATTTTGCCATTCCGCTTCATGGGAACAACAACAATAATAATTACACC
CAGAGAAACTCTGAAGGCTCTAGCCCATGTTATAATGACGACCAAATACGACAACAACAG
CAACCTTTACAAATGCAACCTTTATCAAGAACTTCAAGTTCAAGTGTTAATGTCACAGCG
ATGAGAAGTACATCTGCAGGTAATTCAATTACAGCGAACGCTCCCGTTGTACCTAAGGTG
ATGGTCAATAACCAAAATGTTAAAACTGTTGCTGCCGATCAGTCTGCTACAGCACCTTCT
TCACCTACCATGAATTCGTCCGTCACAACGATCAACCGCGAATCACCATACCAAACCTTA
AAGAAAACGAACAGCACAGGCAATGTTCCTTGTTTGACTGCTGAAAAGACTCATGCGCAC
CCTGCTTTCTATAAACGGGGCAATAATAGCGCCCAAAATTTGACTACCTCTAGTTCAACA
GCTTCTAGGGTGCATCCTATCCGAAAGCACAAGAAAAACGTATCATTTAGTTCTTTGAAT
AGTTTAATGTTTTCAAAAAAGGGTGCAAATCACGGTGGTAATCTAATGACTAACCAATTT
ATGAGTGGTGGAATTCAAGAAGATGATGGTGATTCAACTAATAATGATACTATAAAGCTA
AACCAGTCGATTTATTCTTAA

Protein sequence predicted based on nucleotide sequence

MSDYFSSRPSQTLTPMGNKPSGGGGGDDASSIHSKSSQYLMDILPDSMTLNESVSSIVAN
NQAKEFILPETDERSPYFINVPIPKAQPTSTTETKKPLAGDEAIDGQFVKEYPTDILVDR
FYKWKKILKGLVIYLREVAYAQEQFARINYQLKGSVKFPFLTDIDETTNTITDPFTTAPR
GPKKAQPAQKKVGLTDSEQFQMQMQQEQQENAVQAPTDESKMSLAPHEYKPVQTTESDNT
SAASGFVKFGSGSIQDIQVILKKYHLSLANQQFKISKEITSTVIPKLEELRKDLRYKITE
IKDLHGDFKTNIGAHIQLTSQLLKKYIAAVKFMNAHGIGNDRASPTNKKPHKLDPKHDPY
LLKLQLDLQLKRQVAEETYLQEAFINLQSSGLQLEKIIYTKIQHALLRYSALIDSEARLM
IKNMCQELQHDIISKPPAFEWDNFVTQHPSCLLNWKSNDPIPPPRKVSDVIYPHMKSPLA
KCIKAGYFLKKSELLPTYHQGYFVLTSNYIHEFQSSDFYNLSSSTPNSTKSSAYSSSVSI
ADTYANANNAKANNHHRQASDVHNSSTTTGGTAGANGIRGIRKKSYLAPIMSIPLNDCTL
KDASSTKFVLVGKPTLNENADVRKSSSSTYLSGSSQASLPKYGHETAKIFSKAPFHKFLK
GSKPKNKNTKSSELDQFYAAAQKESNNYVTWTFKIVSPEPSEEELKHFKRWVQDLKNLTS
FNDTKDRIKFIEDRVMKSHRFKAGHMSRNSVNIGSHTPCLTDSTFTLQDGTTTSVNLKGR
AEKPQYIHIQNNSLADFDGNGFRSKVNTPAIDDYGNLITVERRPAQSPHQYSDYMATSGN
TTPSYSSGSGPQSMYNGYNPAVSITSNGMMLQQSTANNNTNPTTNLRHQRNISQTSSLPG
FSYTSLSLPVNSPGSSNSESSSGGYFAIPLHGNNNNNNYTQRNSEGSSPCYNDDQIRQQQ
QPLQMQPLSRTSSSSVNVTAMRSTSAGNSITANAPVVPKVMVNNQNVKTVAADQSATAPS
SPTMNSSVTTINRESPYQTLKKTNSTGNVPCLTAEKTHAHPAFYKRGNNSAQNLTTSSST
ASRVHPIRKHKKNVSFSSLNSLMFSKKGANHGGNLMTNQFMSGGIQEDDGDSTNNDTIKL
NQSIYS*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence 1
End on reference sequence 3441
Mutations:
None
Discrepancy:
t762c; g1292a,G431D;
 a1854g; a2548g,R850G
                                                    

5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable