ECM16 (S. cerevisiae ) in pDONR221 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol: 
Gene Name: 
Original Clone ID:
FLH148260.01X
Keyword:
None
Species:
Saccharomyces cerevisiae
Type:
cDNA
Vector Name:
pDONR221
Format: 
CLOSED
Source:
HIP
Description:
None
Comments:
None
Mutation / Discrepancy:
No / Yes
Publications:
None
Authors:
HIP
ScCD00094123
Price: 
Login for Pricing
AVAILABLE
No restriction
Special MTA: None
Insert sequence: 3804nts
Open reading frame: 1 to 3804

ATGGGTACTTACAGAAAAAGGTTTAATGAAAAAGCCAGATCCGGCCATATGGCCAAATTA
AAAGAACTGAAAAGAATTAGGAATAAGCAGTTCACTAGACAGGATGAAAACGATGAAAGA
GTAGAAAACCCAGATTCAGCTCCCGCTGAGAGTTCTACTACGGAGCCAAATGCCAATGCG
GAAATTCTTGAACCTCTCACTGAAGAAGAAAAGAAAATGAAGAAAAGAAAACTACAGGAG
TTATTTACCCCAAAGGAATCGAAAGTTTCGAGATTGAAGAAGAAAAGACTGGATAAATTT
ATTGAACATCAATTAAAAAGAGAGGAAAGAAAGACAATTATTGGTAAACTTCAAGATTAT
AAGATAGATACAAGTTTGTTGACAAGCTCTAAAAGGTTAGGAGAGGGCAGGCAGACTAAA
AAGGAAGAATTCAAAGAAGCACTGAGTTTAGAACGTCAAGGAAGAGGAAATGAACAAACA
AATGAAATATTATACGAAGAATATGAACCAAAAGTTTGGGATGAGTATGGTGAAGGAGGG
AGCTCTGAGGATGATGACGGCGAAGATGATTTCGAGGCTTCATTTGGTAGTATGCCGAAA
CCAACGGACAATGAGGAGAAGAAATCATCAGGTTTTATTGATCATAGACCAGCAAAATTT
GGTGGATCTGGTCTTAGCTTTGGGTTTAGCAATATAAAAGTCATAAACAAAGAAAGCAAA
ACTCCGAAGAAGAAATATAATTGGAGACAACGAGTAGAAATGGAAGAATTGAAGAAGCAT
GGGAAAGAAGATGAAATGGATTTCGACACGACATCTGAAGATGATGACGAAGAGGAGGAT
CAGGAAGAAGAAGACAAAATGCATCCTAGTGAAAACCCACTCGAAGAAGTTGAAAGCGCA
GATAGTGAAACTGGCTCAGAAAAATTTGACCAAAATGATGTTGCTAACGAATTCAAGGAT
TGGGCCAATCAAGAAATAAAAAAATTGGAAGGTAGAGATCAAGAGCTAGTTACTCCCACT
TTGAACATCGATTATAAACCGATAATTAGAAAGGAAGATTTAGATGATGGTCTCCAAGAA
GCTTATGTTCCAATAAACGAGAATTCCACCCGTAAAGCCTTCTATGTTGAAGTTAGCAGA
TCAGATGAAATTCAGAAAGCAAGAATTCAACTACCAGTATTTGGTGAAGAACACAAAATT
ATGGAGGCTATTCACCATAATGATGTAGTGATTATTTGTGGTGAAACGGGGTCAGGTAAG
ACTACACAAGTCCCACAATTTTTATATGAGGCTGGGTTTGGTGCCGAAGATTCTCCAGAT
TACCCAGGAATGGTGGGTATCACTCAACCAAGAAGAGTGGCCGCTGTGTCCATGGCTGAA
CGTGTAGCTAATGAATTGGGGGACCATGGTCATAAAGTAGGATACCAAATTAGGTTTGAC
TCTACCGCAAAGGAGGACACAAAGGTCAAGTTTATGACAGATGGTGTCTTGCTACGAGAA
ATGATGCACGATTTCAAGTTGACTAAATATTCCTCCATTATTATAGATGAAGCACATGAA
AGAAACATTAACACTGATATTTTGATCGGTATGCTTAGCCGTTGTGTTCGGTTAAGAGCT
AAACTACATAAGGAGAACCCGATCGAGCACAAGAAGCTAAAACTCATCATCATGTCAGCT
ACATTAAGGGTATCAGATTTCAGCGAAAACAAAACATTGTTCCCCATAGCGCCCCCTGTC
TTACAGGTTGATGCAAGGCAGTTTCCAGTCTCTATTCACTTTAATCGTAGAACTGCATTC
AACTATACAGACGAGGCTTTCAGAAAGACTTGCAAAATTCACCAGAAACTTCCTCCTGGC
GCTATATTGGTGTTTTTAACCGGTCAGCAGGAAATTACACACATGGTAAAAAGGCTAAGA
AAGGAATTTCCTTTTAAGAAAAACTCCAAGTACAACAAAGATTTAGAAACACCCGTTTCT
AAAATGGGTATCAATTCAAAAACAACAGATTTGGAGGCTGAGGATATAGACTTCAGTGTA
CAAGTTATCGATCAAGATAAGTTCAAGAGCGCGATAAGATACGAAGAAGATGAAGGTAAC
AGTGGAAACGGTGAAGATGAGGAAGACGAAGAGGAAGAAGGGTTTGAAGAAGTCCTAACT
GAAGGGCAAACAGCTAACGATCCTTTATATGTCCTTCCACTGTATTCTCTATTACCAACC
AAGGAACAAATGAGAGTCTTTCAAAAACCTCCTCAAGGTTCTAGGCTTTGTATTGTTGCA
ACAAATGTTGCAGAAACATCGTTAACTATACCTGGAGTTAGATATGTTGTTGATTCCGGG
AGGTCTAAAGAGCGTAAATATAATGAATCAAATGGCGTACAGAGTTTTGAAGTGGGCTGG
GTAAGTAAAGCTAGTGCCAATCAAAGAAGCGGTAGAGCAGGTCGTACTGGACCAGGCCAT
TGTTACCGTTTATATTCGTCTGCTGTTTTTGAACATGATTTCGAACAGTTCTCTAAACCT
GAAATCTTAAGAATGCCAGTGGAGAGCATCGTGTTACAAATGAAGAGCATGGCCATTCAT
AATATCATTAATTTTCCATTTCCAACTCCTCCAGATAGAGTTGCCCTTTCCAAGGCAATA
CAATTACTGCAATATTTAGGTGCTCTTGATAATAAGGAGATGATTACTGAAGATGGTAAA
AAAATGAGCCTGTTCCCACTTTCACCCAGATTTTCCAAAATGTTACTTGTTTCTGACGAA
AAAGCCTGCCTTCCTTATATTGTAGCAATTGTTAGTGCCCTTTCGGTGGGAGATCCTTTT
ATCAATGAATTTGAGTTGGGAATAAACGAAATTTCTAGAAAACCAAATCCAGATGAAAAT
CTAGATGATAAGATTAGAGAGCACGATGAATCAACACCAGGTATGGATCCTGAACTGAAA
AAGGAATTGCGCAGTAAATTCTATAAAAGCAGGAGTCAGTTTAGTAAATTAGACAAATTT
AGTGACGTATTCCGTTTATTAAGTGTTGTAAGTGCAATGGACTACGTTCCAAAGGAACAA
AAAGAAATATTCATGAAGAAAAATTTTTTAAGAGGCAAACTAATGGAAGAAATAGTAAAA
TTGAGGAAGCAGTTAATGTATATCATCAAGTCCAACACTTCAAAAGAAAATATTGCCGTA
GTAATTAGGAATGAAGACTTGAAAAGTGATATTCCAAGCGTCATACAAATAAAACTTTTA
AAACAAATGATTTGCGCTGGTTTTGTCGACCATGTTGCCGTAAGAGCAGATGTTTTATTT
CCAGATGATGCTAAGATAACCAACAGAACTTCCATAATCAACATTCCTTATATTCCTGTT
TTGGCCACGAGAACACCAAATATCGAGGACTGCTTTGTCTATATCCATCCGACTTCAATT
TTAAATAATTTGGGGGAAATGCCACCCAAATACATGCTCTATTATTCCTTGCACCTTGGT
GGAAATAATAAAACAAGGATGAATACTTTATGTGACATCGCAAGCACCCCCTTAGCGAAC
ATAGCAAGAAAGGGACTTCTTTTGACATACAGTAAACCTCTGACTGGTCAAGGCTTGAAG
ACCGTTAATTTATCACCTACGGAACGGTATTGTTATGTGGTTCCTAGATTTGGATCCACA
GTTGACAACGATCTCAAGATTGGTTGGGATTTAAATCCTATAGCAGTGCATCAGAAGAAG
CAAAAAGGCCAGTGGACAGTCATTAAGTTTATCACCAGAAAGGGCTTCCAGACCATCACA
GGTGAAGAGAAAGAAAAAAAATAG

Protein sequence predicted based on nucleotide sequence

MGTYRKRFNEKARSGHMAKLKELKRIRNKQFTRQDENDERVENPDSAPAESSTTEPNANA
EILEPLTEEEKKMKKRKLQELFTPKESKVSRLKKKRLDKFIEHQLKREERKTIIGKLQDY
KIDTSLLTSSKRLGEGRQTKKEEFKEALSLERQGRGNEQTNEILYEEYEPKVWDEYGEGG
SSEDDDGEDDFEASFGSMPKPTDNEEKKSSGFIDHRPAKFGGSGLSFGFSNIKVINKESK
TPKKKYNWRQRVEMEELKKHGKEDEMDFDTTSEDDDEEEDQEEEDKMHPSENPLEEVESA
DSETGSEKFDQNDVANEFKDWANQEIKKLEGRDQELVTPTLNIDYKPIIRKEDLDDGLQE
AYVPINENSTRKAFYVEVSRSDEIQKARIQLPVFGEEHKIMEAIHHNDVVIICGETGSGK
TTQVPQFLYEAGFGAEDSPDYPGMVGITQPRRVAAVSMAERVANELGDHGHKVGYQIRFD
STAKEDTKVKFMTDGVLLREMMHDFKLTKYSSIIIDEAHERNINTDILIGMLSRCVRLRA
KLHKENPIEHKKLKLIIMSATLRVSDFSENKTLFPIAPPVLQVDARQFPVSIHFNRRTAF
NYTDEAFRKTCKIHQKLPPGAILVFLTGQQEITHMVKRLRKEFPFKKNSKYNKDLETPVS
KMGINSKTTDLEAEDIDFSVQVIDQDKFKSAIRYEEDEGNSGNGEDEEDEEEEGFEEVLT
EGQTANDPLYVLPLYSLLPTKEQMRVFQKPPQGSRLCIVATNVAETSLTIPGVRYVVDSG
RSKERKYNESNGVQSFEVGWVSKASANQRSGRAGRTGPGHCYRLYSSAVFEHDFEQFSKP
EILRMPVESIVLQMKSMAIHNIINFPFPTPPDRVALSKAIQLLQYLGALDNKEMITEDGK
KMSLFPLSPRFSKMLLVSDEKACLPYIVAIVSALSVGDPFINEFELGINEISRKPNPDEN
LDDKIREHDESTPGMDPELKKELRSKFYKSRSQFSKLDKFSDVFRLLSVVSAMDYVPKEQ
KEIFMKKNFLRGKLMEEIVKLRKQLMYIIKSNTSKENIAVVIRNEDLKSDIPSVIQIKLL
KQMICAGFVDHVAVRADVLFPDDAKITNRTSIINIPYIPVLATRTPNIEDCFVYIHPTSI
LNNLGEMPPKYMLYYSLHLGGNNKTRMNTLCDIASTPLANIARKGLLLTYSKPLTGQGLK
TVNLSPTERYCYVVPRFGSTVDNDLKIGWDLNPIAVHQKKQKGQWTVIKFITRKGFQTIT
GEEKEKK*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence 1
End on reference sequence 3804
Mutations:
None
Discrepancy:
c1542t
                                                    

5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable