ORC1 (S. cerevisiae ) in pDONR221 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol: 
Gene Name: 
Original Clone ID:
FLH237603.01X
Keyword:
None
Species:
Saccharomyces cerevisiae
Type:
cDNA
Vector Name:
pDONR221
Format: 
CLOSED
Source:
HIP
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
HIP
ScCD00094189
Price: 
Login for Pricing
AVAILABLE
No restriction
Special MTA: None
Insert sequence: 2745nts
Open reading frame: 1 to 2745

ATGGCAAAAACGTTGAAGGATTTACAGGGTTGGGAGATAATAACAACTGATGAGCAGGGA
AATATAATCGATGGAGGTCAGAAGAGATTACGCCGAAGAGGTGCAAAAACTGAACATTAC
TTAAAGAGAAGTTCTGATGGAATTAAACTAGGTCGTGGTGATAGTGTAGTCATGCACAAC
GAAGCCGCTGGGACTTACTCCGTTTATATGATCCAGGAGTTGAGACTTAATACATTAAAT
AATGTTGTCGAACTCTGGGCTCTCACCTATTTACGATGGTTTGAAGTCAATCCTTTAGCT
CATTATAGGCAGTTTAATCCTGACGCTAACATTTTGAATCGTCCTTTAAATTATTACAAT
AAACTGTTTTCTGAAACTGCAAATAAAAATGAACTGTATCTCACTGCAGAATTAGCCGAA
TTGCAGCTATTTAACTTTATCAGGGTTGCCAACGTAATGGATGGAAGCAAATGGGAAGTA
TTGAAAGGAAATGTCGATCCAGAAAGAGACTTTACAGTTCGTTATATTTGTGAGCCGACT
GGGGAGAAATTTGTGGACATTAATATTGAGGATGTCAAAGCTTACATAAAGAAAGTGGAG
CCAAGGGAAGCCCAGGAATATTTGAAAGATTTAACACTTCCATCAAAGAAGAAAGAGATC
AAAAGAGGTCCTCAAAAGAAAGATAAGGCTACTCAAACGGCACAAATTTCAGACGCAGAA
ACAAGAGCTACAGATATAACGGATAATGAGGACGGTAATGAAGATGAATCATCTGATTAT
GAAAGTCCGTCAGATATCGACGTTAGCGAGGATATGGACAGCGGTGAAATATCCGCAGAT
GAGCTTGAGGAAGAAGAAGACGAAGAAGAAGACGAAGACGAAGAAGAGAAAGAAGCTAGG
CATACAAATTCACCAAGGAAAAGAGGCCGTAAGATAAAACTAGGTAAAGATGATATTGAC
GCTTCTGTACAACCTCCCCCCAAAAAAAGAGGTCGTAAACCTAAAGATCCTAGTAAACCG
CGTCAGATGCTATTGATATCTTCATGCCGTGCAAATAATACTCCTGTGATTAGGAAATTT
ACAAAAAAGAATGTTGCTAGGGCGAAAAAGAAATATACCCCGTTTTCGAAAAGATTTAAA
TCTATAGCTGCAATACCAGATTTAACTTCATTACCTGAATTTTACGGAAATTCTTCGGAA
TTGATGGCATCAAGGTTTGAAAACAAATTAAAAACAACCCAAAAGCATCAGATTGTAGAA
ACAATTTTTTCTAAAGTCAAAAAACAGTTGAACTCTTCGTATGTCAAAGAAGAAATATTG
AAGTCTGCAAATTTCCAAGATTATTTACCGGCTAGGGAGAATGAATTCGCCTCAATTTAT
TTAAGTGCATATAGTGCCATTGAGTCCGACTCCGCTACTACTATATACGTGGCTGGTACG
CCTGGTGTAGGGAAAACTTTAACCGTAAGGGAAGTCGTAAAGGAACTACTATCGTCTTCT
GCACAACGAGAAATACCAGACTTTCTTTATGTGGAAATAAATGGATTGAAAATGGTAAAA
CCCACAGACTGTTACGAAACTTTATGGAACAAAGTGTCAGGAGAAAGGTTAACATGGGCA
GCTTCAATGGAGTCACTAGAGTTTTACTTTAAAAGAGTTCCAAAAAATAAGAAGAAAACC
ATTGTAGTCTTGTTGGACGAACTCGATGCCATGGTAACGAAATCTCAAGATATTATGTAC
AATTTTTTCAATTGGACTACTTACGAAAATGCCAAACTTATTGTCATTGCAGTAGCCAAT
ACAATGGACTTACCAGAACGTCAGCTAGGCAATAAGATTACTTCAAGAATTGGGTTTACC
AGAATTATGTTCACTGGGTATACGCACGAAGAGCTAAAAAATATCATTGATTTAAGACTG
AAGGGGTTGAACGACTCATTTTTCTATGTTGATACAAAAACTGGCAATGCTATTTTGATT
GATGCGGCTGGAAACGACACTACAGTTAAGCAAACGTTGCCTGAAGACGTGAGGAAAGTT
CGCTTAAGAATGAGTGCTGATGCCATTGAAATAGCTTCGAGAAAAGTAGCAAGTGTTAGT
GGTGATGCAAGAAGAGCATTGAAGGTTTGTAAAAGAGCAGCTGAAATTGCTGAAAAACAC
TATATGGCTAAGCATGGTTATGGATATGATGGAAAGACGGTTATTGAAGATGAAAATGAG
GAGCAAATATACGATGATGAAGACAAGGATCTTATTGAAAGTAACAAAGCCAAAGACGAT
AATGATGACGATGATGACAATGATGGGGTACAAACAGTTCACATCACGCACGTTATGAAA
GCCTTAAACGAAACTTTAAATTCTCATGTAATTACGTTTATGACGCGACTTTCATTTACA
GCAAAACTGTTTATTTATGCATTATTAAACTTGATGAAAAAGAACGGATCTCAAGAGCAA
GAACTGGGCGATATTGTCGATGAAATCAAGTTACTTATTGAAGTAAATGGCAGTAATAAG
TTTGTCATGGAGATAGCCAAAACATTGTTCCAACAGGGAAGTGATAATATTTCTGAACAA
TTGAGAATTATATCATGGGATTTCGTTCTCAATCAGTTACTTGACGCGGGAATATTGTTT
AAACAAACTATGAAGAACGATAGAATATGTTGTGTCAAGCTAAATATATCAGTAGAAGAA
GCCAAAAGAGCCATGAATGAGGATGAGACATTGAGAAATTTATAG

Protein sequence predicted based on nucleotide sequence

MAKTLKDLQGWEIITTDEQGNIIDGGQKRLRRRGAKTEHYLKRSSDGIKLGRGDSVVMHN
EAAGTYSVYMIQELRLNTLNNVVELWALTYLRWFEVNPLAHYRQFNPDANILNRPLNYYN
KLFSETANKNELYLTAELAELQLFNFIRVANVMDGSKWEVLKGNVDPERDFTVRYICEPT
GEKFVDINIEDVKAYIKKVEPREAQEYLKDLTLPSKKKEIKRGPQKKDKATQTAQISDAE
TRATDITDNEDGNEDESSDYESPSDIDVSEDMDSGEISADELEEEEDEEEDEDEEEKEAR
HTNSPRKRGRKIKLGKDDIDASVQPPPKKRGRKPKDPSKPRQMLLISSCRANNTPVIRKF
TKKNVARAKKKYTPFSKRFKSIAAIPDLTSLPEFYGNSSELMASRFENKLKTTQKHQIVE
TIFSKVKKQLNSSYVKEEILKSANFQDYLPARENEFASIYLSAYSAIESDSATTIYVAGT
PGVGKTLTVREVVKELLSSSAQREIPDFLYVEINGLKMVKPTDCYETLWNKVSGERLTWA
ASMESLEFYFKRVPKNKKKTIVVLLDELDAMVTKSQDIMYNFFNWTTYENAKLIVIAVAN
TMDLPERQLGNKITSRIGFTRIMFTGYTHEELKNIIDLRLKGLNDSFFYVDTKTGNAILI
DAAGNDTTVKQTLPEDVRKVRLRMSADAIEIASRKVASVSGDARRALKVCKRAAEIAEKH
YMAKHGYGYDGKTVIEDENEEQIYDDEDKDLIESNKAKDDNDDDDDNDGVQTVHITHVMK
ALNETLNSHVITFMTRLSFTAKLFIYALLNLMKKNGSQEQELGDIVDEIKLLIEVNGSNK
FVMEIAKTLFQQGSDNISEQLRIISWDFVLNQLLDAGILFKQTMKNDRICCVKLNISVEE
AKRAMNEDETLRNL*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence 1
End on reference sequence 2745
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable