SEC31 (S. cerevisiae ) in pDONR221 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol: 
Gene Name: 
Original Clone ID:
FLH237664.01X
Keyword:
None
Species:
Saccharomyces cerevisiae
Type:
cDNA
Vector Name:
pDONR221
Format: 
CLOSED
Source:
HIP
Description:
None
Comments:
None
Mutation / Discrepancy:
No / Yes
Publications:
None
Authors:
HIP
ScCD00094199
Price: 
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 3822nts
Open reading frame: 1 to 3822

ATGGTCAAACTTGCTGAGTTTTCTCGAACAGCCACGTTTGCGTGGTCACATGATAAAATT
CCATTATTGGTCTCTGGTACCGTATCTGGTACGGTGGATGCTAATTTCTCCACTGATTCA
TCTCTAGAATTGTGGTCATTGTTGGCTGCTGATTCGGAGAAGCCTATTGCTTCCTTGCAA
GTGGATTCCAAATTCAATGATTTGGATTGGTCTCATAATAACAAGATTATTGCTGGTGCT
CTGGATAACGGTAGTTTGGAATTGTACTCCACCAATGAAGCAAACAACGCTATCAACTCC
ATGGCCAGATTTAGCAACCATTCTTCCTCTGTGAAGACGGTAAAGTTTAACGCAAAGCAA
GACAACGTTCTTGCTTCGGGTGGTAACAACGGTGAAATTTTTATTTGGGACATGAATAAA
TGCACTGAATCGCCCTCCAATTATACTCCATTGACACCGGGTCAATCGATGTCGTCCGTT
GACGAGGTCATTTCCCTAGCATGGAACCAATCTTTGGCCCATGTTTTTGCATCTGCCGGG
TCGTCTAATTTCGCATCTATTTGGGATTTGAAGGCTAAGAAGGAAGTCATTCATCTAAGT
TACACTTCACCTAATTCAGGTATCAAGCAACAGCTGTCCGTTGTTGAATGGCACCCAAAA
AACTCCACAAGAGTGGCAACGGCTACTGGTAGCGATAATGATCCATCTATCCTGATCTGG
GATTTAAGAAACGCCAACACACCATTGCAGACTTTAAATCAAGGCCATCAAAAGGGTATT
TTGTCATTAGATTGGTGTCATCAGGACGAACATCTATTATTGTCCAGTGGTAGAGATAAT
ACCGTTCTTCTATGGAACCCTGAGTCAGCCGAACAACTGTCCCAATTCCCAGCTCGTGGA
AACTGGTGTTTTAAGACCAAATTTGCACCAGAGGCTCCAGACCTATTTGCTTGTGCCTCC
TTTGATAACAAAATTGAGGTACAGACTTTGCAAAATCTCACAAACACTTTGGATGAGCAA
GAAACCGAAACTAAGCAGCAAGAATCTGAAACAGATTTTTGGAATAATGTTTCCCGAGAG
GAATCAAAAGAGAAGCCAACTGTTTTCCATTTACAAGCCCCAACTTGGTATGGGGAACCA
TCTCCCGCAGCTCATTGGGCTTTCGGTGGTAAATTGGTTCAAATTACTCCAGATGGTAAA
GGTGTATCTATAACAAACCCAAAAATTTCAGGCTTAGAATCAAACACTACTTTGAGTGAA
GCGTTGAAAACTAAGGATTTCAAACCATTAATAAATCAAAGACTGGTCAAAGTTATTGAT
GACGTTAATGAAGAAGATTGGAATTTATTGGAAAAGTTATCAATGGACGGTACTGAGGAG
TTCTTGAAAGAGGCTCTTGCATTCGACAACGATGAATCAGATGCACAAGACGATGCCAAC
AATGAGAAAGAAGACGATGGGGAAGAATTCTTTCAACAAATTGAAACCAATTTCCAACCC
GAGGGCGATTTCTCCTTGTCTGGTAATATCGAACAAACTATTTCCAAGAACTTGGTTTCT
GGCAACATTAAGAGCGCTGTGAAAAATTCTCTAGAGAATGACTTACTAATGGAGGCCATG
GTGATCGCATTAGATTCAAATAACGAAAGATTAAAGGAAAGTGTCAAGAATGCCTATTTT
GCGAAGTATGGATCTAAATCATCGCTCTCGAGGATACTATACTCCATTTCTAAGAGGGAA
GTAGATGATTTGGTTGAAAATTTGGATGTCTCTCAGTGGAAGTTTATCTCTAAAGCAATT
CAAAACTTATATCCAAATGATATCGCCCAGAGGAATGAAATGTTGATTAAATTGGGAGAC
AGGTTAAAGGAAAATGGTCATAGACAAGATTCTTTGACTTTGTACTTGGCTGCCGGATCA
TTAGATAAGGTGGCTTCAATTTGGTTATCAGAATTTCCAGATTTGGAGGATAAATTGAAG
AAAGATAATAAGACAATTTATGAAGCTCATTCCGAATGTCTAACTGAGTTCATTGAAAGA
TTCACCGTATTTTCCAACTTCATTAATGGAAGCTCTACCATTAATAATGAGCAATTAATT
GCCAAATTTTTGGAATTTATCAACTTAACTACTTCCACAGGAAATTTCGAACTAGCCACT
GAATTCTTAAATAGTTTACCAAGTGACAATGAAGAGGTTAAAACAGAAAAGGCACGTGTC
TTGATTGCTTCCGGCAAATCATTACCGGCACAAAATCCTGCGACAGCGACGACCAGCAAA
GCCAAGTATACAAACGCCAAGACAAATAAGAACGTTCCTGTACTACCAACTCCTGGAATG
CCTTCTACTACTTCTATTCCTAGTATGCAGGCACCATTTTATGGTATGACACCAGGCGCC
TCTGCAAATGCTCTACCTCCAAAGCCGTACGTTCCAGCAACCACCACTAGTGCTCCTGTT
CATACAGAAGGTAAATATGCGCCACCAAGCCAACCTTCGATGGCGTCACCTTTTGTTAAC
AAAACAAATAGCTCGACCAGATTGAATTCTTTTGCTCCTCCGCCTAACCCATATGCCACT
GCAACAGTTCCTGCAACGAACGTATCTACAACGTCGATTCCGCAAAACACTTTTGCTCCT
ATACAACCTGGTATGCCTATTATGGGCGACTATAATGCTCAATCTAGCTCTATTCCTTCA
CAACCTCCAATTAATGCTGTATCGGGTCAAACGCCACATCTCAACCGTAAAGCCAATGAT
GGTTGGAATGATTTGCCTTTGAAGGTCAAAGAAAAACCATCTCGTGCCAAGGCTGTATCT
GTTGCCCCTCCAAATATCCTATCGACACCAACTCCATTAAATGGTATCCCTGCAAATGCT
GCTAGTACCATGCCTCCGCCACCTCTTTCCAGAGCTCCCTCTTCTGTGTCAATGGTATCA
CCACCTCCTCTACACAAAAATTCTAGAGTCCCATCCTTGGTTGCAACTTCTGAGTCACCA
AGGGCATCCATATCAAATCCATACGCTCCTCCTCAATCATCACAACAATTCCCAATAGGT
ACTATTTCTACAGCAAACCAAACGTCAAACACCGCTCAGGTAGCTTCATCGAACCCCTAT
GCTCCACCACCACAACAAAGAGTAGCAACCCCATTATCTGGAGGCGTGCCTCCAGCTCCG
TTGCCAAAGGCCTCTAATCCATATGCTCCAACTGCAACCACTCAACCCAACGGTTCCTCC
TATCCTCCAACCGGTCCGTATACTAATAACCATACCATGACCTCTCCTCCTCCCGTTTTT
AACAAACCTCCCACTGGCCCCCCTCCGATTAGCATGAAGAAGAGAAGCAACAAGTTAGCT
AGTATAGAACAAAACCCATCTCAAGGTGCTACTTATCCTCCAACCCTTTCCAGCTCGGCC
TCTCCATTGCAGCCTTCTCAACCGCCAACTTTGGCTTCTCAGGTTAATACCTCCGCTGAG
AATGTCAGTCATGAAATTCCAGCTGATCAACAACCCATTGTCGACTTCTTGAAAGAAGAA
CTGGCTCGCGTAACACCATTGACCCCAAAGGAGTACTCCAAACAATTAAAGGATTGTGAT
AAACGATTAAAGATTCTTTTCTACCATTTGGAAAAGCAGGATTTATTAACCCAACCAACA
ATCGATTGTTTACATGACCTCGTCGCATTAATGAAGGAAAAGAAATACAAAGAAGCTATG
GTCATCCATGCTAATATCGCTACAAACCATGCTCAAGAGGGTGGTAACTGGCTGACAGGA
GTGAAGAGGTTGATTGGCATAGCTGAAGCGACTTTGAATTAG

Protein sequence predicted based on nucleotide sequence

MVKLAEFSRTATFAWSHDKIPLLVSGTVSGTVDANFSTDSSLELWSLLAADSEKPIASLQ
VDSKFNDLDWSHNNKIIAGALDNGSLELYSTNEANNAINSMARFSNHSSSVKTVKFNAKQ
DNVLASGGNNGEIFIWDMNKCTESPSNYTPLTPGQSMSSVDEVISLAWNQSLAHVFASAG
SSNFASIWDLKAKKEVIHLSYTSPNSGIKQQLSVVEWHPKNSTRVATATGSDNDPSILIW
DLRNANTPLQTLNQGHQKGILSLDWCHQDEHLLLSSGRDNTVLLWNPESAEQLSQFPARG
NWCFKTKFAPEAPDLFACASFDNKIEVQTLQNLTNTLDEQETETKQQESETDFWNNVSRE
ESKEKPTVFHLQAPTWYGEPSPAAHWAFGGKLVQITPDGKGVSITNPKISGLESNTTLSE
ALKTKDFKPLINQRLVKVIDDVNEEDWNLLEKLSMDGTEEFLKEALAFDNDESDAQDDAN
NEKEDDGEEFFQQIETNFQPEGDFSLSGNIEQTISKNLVSGNIKSAVKNSLENDLLMEAM
VIALDSNNERLKESVKNAYFAKYGSKSSLSRILYSISKREVDDLVENLDVSQWKFISKAI
QNLYPNDIAQRNEMLIKLGDRLKENGHRQDSLTLYLAAGSLDKVASIWLSEFPDLEDKLK
KDNKTIYEAHSECLTEFIERFTVFSNFINGSSTINNEQLIAKFLEFINLTTSTGNFELAT
EFLNSLPSDNEEVKTEKARVLIASGKSLPAQNPATATTSKAKYTNAKTNKNVPVLPTPGM
PSTTSIPSMQAPFYGMTPGASANALPPKPYVPATTTSAPVHTEGKYAPPSQPSMASPFVN
KTNSSTRLNSFAPPPNPYATATVPATNVSTTSIPQNTFAPIQPGMPIMGDYNAQSSSIPS
QPPINAVSGQTPHLNRKANDGWNDLPLKVKEKPSRAKAVSVAPPNILSTPTPLNGIPANA
ASTMPPPPLSRAPSSVSMVSPPPLHKNSRVPSLVATSESPRASISNPYAPPQSSQQFPIG
TISTANQTSNTAQVASSNPYAPPPQQRVATPLSGGVPPAPLPKASNPYAPTATTQPNGSS
YPPTGPYTNNHTMTSPPPVFNKPPTGPPPISMKKRSNKLASIEQNPSQGATYPPTLSSSA
SPLQPSQPPTLASQVNTSAENVSHEIPADQQPIVDFLKEELARVTPLTPKEYSKQLKDCD
KRLKILFYHLEKQDLLTQPTIDCLHDLVALMKEKKYKEAMVIHANIATNHAQEGGNWLTG
VKRLIGIAEATLN*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence 1
End on reference sequence 3822
Mutations:
None
Discrepancy:
t1099a,S367T
                                                    

5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable