BEM3 (S. cerevisiae ) in pBY011 (yeast expression vector)

Explanation of Terms

Gene:
Gene Symbol: 
Gene Name: 
Original Clone ID:
FLH228229.01X
Keyword:
None
Species:
Saccharomyces cerevisiae
Type:
cDNA
Vector Name:
pBY011
Format: 
CLOSED
Source:
HIP
Description:
None
Comments:
None
Mutation / Discrepancy:
No / Yes
Publications:
None
Authors:
ScCD00094656
Price: 
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 3387nts
Open reading frame: 1 to 3387

ATGACAGATAATTTGACCACAACTCATGGTGGTAGTACCACCCTTGAATTGTTGGCGCAG
TACAATGATCATAGATCCAAAAAGGACAAATCTATAGAACATATAGAAAAAGGAACATGT
TCAGGAAAAGAAAGAAATCCGTCCTATGATGAAATTTTTACGGAAAACATTAAGCTAAAG
CTACAAGTCCAAGAATATGAAACTGAAATTGAGAGTTTGGAAAAGGTTATTGATATGTTA
CAAAAAAACAGAGAAGCTTCCTTAGAAGTTGTGCTTGAGCAAGTTCAAAATGACTCAAGA
GATTCATACGTTAACGATCAATCATTTGTGTTACCCCCAAGATCTGCTGAAAGGAAAGCG
CATATCAAGAGTCTTAATTTGCCAATACCGACATTGTCCCCTCCATTGCAACAAGGTTCA
GACGTTGCACTTGAAACAAGTGTAACGCCTACAGTACCTCAAATAGGTGTTACATCAAAT
ACTTCAATAAGTAGGAAGCATTTACAGAACATGATACTAAACGATGAAATAGAAGCAAAT
AGTAGCTTCTCATCTCCCAAAATTATCAATAGATCTGTCTCAAGCCCTACAAAAATACAT
TCAGAACAGTTGGCTAGCCCAGCTGCCTCTGTTACCTATACGACGTCTAGGATAACCATA
AAATCACCTAATAAAGGTTCGAAATCTCCATTGCAAGAACGTTTGCGTTCCCCTCAAAAT
CCTAACAGAATGACGGCTGTTATCAACAACCATTTGCATTCTCCATTAAAGGCCAGTACC
TCTAATAATTTAGATGAGCTGACTGAAAGTAAAAGTCAACAACTAACTAATGACGCGATA
CAAAAAAATGACCGCGTTTATTCTTCTATAACATCCTCTGCATACACTACTGGAACTCCT
ACTTCAGCAGCCAAAAGTCCATCATCCCTTTTGGAAGTCAAGGAGGGTGAAAACAAGGCT
TTGGGTTTCTCTCCAGCATCTAAAGAAAAACTTGACGACTTCACCCAATTACTTGACTCC
TCATTTGGCGAAGAGGATCTAGTCAATACGGACAGTAAAGATCCTCTGTCCATAAAATCC
ACGATTAACGAATCTTTACCCCCACCTCCAGCGCCTCCAACTTTTTTCTCACCTACTTCT
AGTGGAAATATAAAAAACTCTACTCCTTTATCAAGCCATTTGGCTTCTCCGGTTATCTTG
AATAAAAAGGATGATAATTTTGGTGCTCAAAGTGCAAAAAATTTAAAGAAACCTGTGTTA
ACTTCATCACTCCCCAATCTTTCTACAAAATTGTCAACAACAAGTCAAAATGCTTCTCTT
CCTCCAAATCCTCCCGTGGAAAGTTCATCAAAACAAAAACAACTTGGAGAAACGGCATCC
ATCCATTCAACCAATACCTTGAATACATTTAGCTCAACACCCCAAGGTTCGTTGAAGACA
CTTAGAAGACCTCATGCAAGTAGCGTGAGCACCGTGAAGTCTGTGGCCCAAAGTCTTAAG
TCGGATATTCCATTATTCGTTCAACCGGAGGATTTTGGCACTATCCAGATTGAAGTACTG
AGCACATTGTACAGGGATAATGAAGATGATTTATCCATTTTAATTGCAATCATAGACAGG
AAATCCGGTAAGGAGATGTTTAAATTTTCAAAATCTATTCATAAAGTTCGCGAATTAGAT
GTATATATGAAATCACATGTTCCCGATTTACCATTGCCCACGTTACCTGATAGACAATTA
TTTCAGACTTTATCACCTACAAAAGTGGATACGCGAAAGAACATATTGAATCAGTATTAT
ACAAGCATATTCAGTGTCCCTGAATTTCCCAAAAATGTTGGTTTAAAAATCGCCCAATTT
ATTAGTACAGATACAGTAATGACACCTCCAATGATGGATGATAATGTGAAAGACGGATCA
CTTTTGCTGAGAAGACCAAAGACTCTAACAGGTAATAGTACATGGAGAGTTCGGTACGGT
ATTTTGCGCGATGATGTTTTACAATTGTTTGACAAAAATCAGTTGACTGAAACTATTAAA
TTGAGACAATCTTCTATAGAACTTATACCCAATCTCCCTGAAGATAGATTTGGAACGAGA
AATGGGTTTTTGATCACTGAGCATAAGAAAAGCGGACTGTCAACCTCAACAAAGTATTAT
ATTTGTACTGAAACGTCGAAGGAACGTGAATTATGGCTTTCTGCGTTTAGTGATTACATT
GATCCTTCACAAAGTTTATCGTTATCTAGCTCGAGGAATGCAAACGATACTGATTCTGCA
TCTCACTTGAGCGCAGGAACACACCATTCTAAATTTGGAAACGCAACTATCTCAGCAACC
GATACTCCATCATATGTCACTGATTTGACCCAAGAATATAATAATAATAACAATATTAGT
AATAGTAGCAATAACATTGCTAATAGCGATGGTATTGACTCAAACCCATCTTCTCACTCT
AACTTCCTAGCAAGTTCAAGCGGGAATGCAGAGGAGGAGAAGGATAGTAGGAGAGCCAAA
ATGAGAAGTTTATTCCCTTTTAAAAAATTAACCGGCCCGGCGAGTGCAATGAACCATATA
GGTATTACAATTTCAAATGATTCGGATTCTCCAACATCTCCTGACTCTATCATTAAGTCA
CCAAGCAAAAAATTAATGGAAGTATCCTCTTCGTCAAATTCTTCTACGGGTCCACATGTG
TCCACTGCAATATTTGGCTCGTCACTAGAAACGTGTTTAAGATTGAGTTCGCATAAGTAC
CAAAACGTTTACGATTTACCGAGTGTAGTTTATCGTTGCTTAGAGTATCTTTACAAAAAC
CGTGGTATCCAGGAAGAAGGCATATTTAGACTAAGCGGGTCCAGTACAGTTATCAAAACT
TTACAAGAAAGGTTTGATAAAGAGTACGATGTAGATTTATGTCGGTACAATGAAAGTATT
GAAGCTAAAGATGATGAGGCATCTCCAAGTCTTTACATTGGTGTGAATACTGTAAGTGGG
TTACTAAAGTTGTACTTGAGAAAGTTGCCCCATTTATTATTTGGTGACGAACAATTTTTG
TCGTTTAAAAGAGTAGTTGATGAAAATCATAATAATCCTGTTCAAATATCATTGGGGTTT
AAGGAACTTATTGAATCTGGCCTAGTTCCTCACGCTAACCTATCGTTGATGTATGCTCTT
TTTGAACTGCTGGTAAGGATCAATGAGAATAGTAAGTTTAATAAGATGAATCTAAGAAAT
TTATGCATCGTGTTTTCCCCAACTCTAAATATTCCAATTAGTATGCTTCAACCATCCATC
ACTGACTTTGCGTGTATATTTCAGGGTGGTGAACCAGTTAAAGAAGAGGAAAGGGAGAAA
GTAGATATACATATTCCTCAGGTTTGA

Protein sequence predicted based on nucleotide sequence

MTDNLTTTHGGSTTLELLAQYNDHRSKKDKSIEHIEKGTCSGKERNPSYDEIFTENIKLK
LQVQEYETEIESLEKVIDMLQKNREASLEVVLEQVQNDSRDSYVNDQSFVLPPRSAERKA
HIKSLNLPIPTLSPPLQQGSDVALETSVTPTVPQIGVTSNTSISRKHLQNMILNDEIEAN
SSFSSPKIINRSVSSPTKIHSEQLASPAASVTYTTSRITIKSPNKGSKSPLQERLRSPQN
PNRMTAVINNHLHSPLKASTSNNLDELTESKSQQLTNDAIQKNDRVYSSITSSAYTTGTP
TSAAKSPSSLLEVKEGENKALGFSPASKEKLDDFTQLLDSSFGEEDLVNTDSKDPLSIKS
TINESLPPPPAPPTFFSPTSSGNIKNSTPLSSHLASPVILNKKDDNFGAQSAKNLKKPVL
TSSLPNLSTKLSTTSQNASLPPNPPVESSSKQKQLGETASIHSTNTLNTFSSTPQGSLKT
LRRPHASSVSTVKSVAQSLKSDIPLFVQPEDFGTIQIEVLSTLYRDNEDDLSILIAIIDR
KSGKEMFKFSKSIHKVRELDVYMKSHVPDLPLPTLPDRQLFQTLSPTKVDTRKNILNQYY
TSIFSVPEFPKNVGLKIAQFISTDTVMTPPMMDDNVKDGSLLLRRPKTLTGNSTWRVRYG
ILRDDVLQLFDKNQLTETIKLRQSSIELIPNLPEDRFGTRNGFLITEHKKSGLSTSTKYY
ICTETSKERELWLSAFSDYIDPSQSLSLSSSRNANDTDSASHLSAGTHHSKFGNATISAT
DTPSYVTDLTQEYNNNNNISNSSNNIANSDGIDSNPSSHSNFLASSSGNAEEEKDSRRAK
MRSLFPFKKLTGPASAMNHIGITISNDSDSPTSPDSIIKSPSKKLMEVSSSSNSSTGPHV
STAIFGSSLETCLRLSSHKYQNVYDLPSVVYRCLEYLYKNRGIQEEGIFRLSGSSTVIKT
LQERFDKEYDVDLCRYNESIEAKDDEASPSLYIGVNTVSGLLKLYLRKLPHLLFGDEQFL
SFKRVVDENHNNPVQISLGFKELIESGLVPHANLSLMYALFELLVRINENSKFNKMNLRN
LCIVFSPTLNIPISMLQPSITDFACIFQGGEPVKEEEREKVDIHIPQV*

Coding Sequence Details

Insert Sequence Verified?:
N
Verification Method:
Insert was fully sequenced in parent vector
Reference Sequence Annotations:
Start on reference sequence 1
End on reference sequence 3387
Mutations:
None
Discrepancy:
t3296c,F1099S
                                                    

5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  yeast    Marker:  URA
Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition: 100 ug/mL ampicillin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable