Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
FLH261715.01X
Keyword:
None
Species:
Saccharomyces cerevisiae
Type:
cDNA
Vector Name:
pBY011
Format:
CLOSED
Source:
HIP
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
ScCD00095721
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 4995nts
Open reading frame: 1 to 4995
ATGGATATTTCTAAACCGGTTGGTTCTGAAATCACATCCGTTGATTTCGGGATCCTAACA
GCTAAAGAGATTAGAAATCTATCAGCAAAGCAAATTACCAATCCTACGGTTTTAGATAAC
TTAGGTCATCCAGTATCAGGCGGTTTATATGACTTAGCCTTAGGTGCATTCTTAAGGAAT
CTGTGTTCTACTTGTGGTTTGGATGAAAAATTCTGTCCAGGTCACCAAGGTCACATTGAA
TTACCTGTCCCTTGTTATAATCCATTGTTCTTTAACCAGCTATATATTTATTTGAGGGCA
TCATGTTTATTCTGTCATCATTTCCGTTTAAAGTCAGTAGAGGTTCATCGTTACGCATGT
AAATTGAGATTGTTACAATACGGTTTGATAGACGAATCTTACAAACTAGATGAAATAACT
TTGGGCTCTCTTAATAGCAGTATGTACACTGACGACGAAGCTATTGAAGATAATGAGGAT
GAAATGGATGGTGAAGGAAGCAAGCAATCAAAGGATATTTCCTCCACTCTCTTAAATGAA
CTGAAAAGTAAGCGTTCAGAATATGTTGACATGGCCATCGCTAAAGCTTTGTCAGACGGC
AGAACTACTGAGAGAGGCTCTTTCACAGCTACTGTAAATGATGAACGAAAGAAATTGGTC
CATGAGTTTCACAAAAAACTATTATCTAGAGGTAAGTGTGATAACTGTGGTATGTTTTCG
CCAAAATTTAGAAAGGATGGTTTTACAAAAATCTTCGAAACTGCGTTGAATGAAAAACAA
ATTACAAATAACAGAGTAAAAGGTTTCATTCGTCAAGATATGATTAAGAAGCAAAAACAG
GCTAAGAAATTGGACGGTTCTAACGAAGCATCTGCAAATGACGAAGAAAGTTTTGACGTT
GGTAGAAATCCTACCACCAGACCAAAAACCGGCTCAACATATATTTTATCTACTGAAGTG
AAGAATATTCTGGACACTGTTTTCAGGAAGGAGCAATGTGTTTTACAATATGTTTTTCAT
TCGAGACCCAATTTATCAAGAAAATTGGTCAAGGCTGACTCTTTTTTTATGGATGTTCTT
GTTGTGCCACCAACTAGATTCCGTTTGCCTTCCAAGCTTGGTGAAGAAGTTCATGAAAAC
TCTCAAAACCAATTGTTGTCCAAGGTTCTTACTACGTCATTATTAATTAGGGACTTAAAT
GATGATTTATCTAAGCTACAAAAGGACAAAGTTTCTTTGGAAGATAGAAGGGTGATCTTC
AGTAGGTTAATGAATGCCTTTGTTACTATTCAAAATGACGTCAATGCTTTTATCGACTCT
ACCAAAGCACAAGGTAGAACCAGTGGTAAGGTTCCAATTCCTGGTGTTAAACAAGCACTG
GAAAAGAAAGAAGGTTTATTCAGAAAACATATGATGGGTAAGCGTGTTAACTATGCCGCT
CGTTCTGTTATTTCTCCAGACCCAAATATCGAAACTAATGAAATTGGTGTCCCACCTGTT
TTTGCTGTTAAATTGACTTATCCAGAACCAGTAACGGCTTATAACATAGCAGAGTTGCGT
CAAGCTGTTATTAATGGCCCTGATAAATGGCCAGGTGCCACTCAAATACAGAACGAAGAT
GGTTCTTTGGTCTCTTTGATCGGTATGTCTGTAGAGCAACGTAAGGCATTAGCTAACCAA
CTATTGACACCATCTTCAAACGTTTCTACTCATACTTTGAATAAAAAGGTTTACCGTCAC
ATCAAGAACAGAGATGTTGTTTTAATGAACCGTCAACCGACCTTACATAAAGCCTCCATG
ATGGGTCACAAAGTTAGGGTGTTACCAAACGAAAAGACTTTGAGATTACATTATGCTAAT
ACAGGTGCTTATAATGCAGATTTCGATGGTGATGAAATGAACATGCATTTTCCACAAAAC
GAAAATGCTAGAGCAGAAGCACTAAACTTAGCTAATACTGATTCTCAATACTTGACACCA
ACTTCTGGTTCTCCAGTTAGAGGTTTGATTCAAGATCATATTTCTGCGGGTGTTTGGCTA
ACAAGTAAGGACAGTTTTTTCACCAGAGAACAATACCAGCAATATATTTACGGTTGCATC
CGTCCTGAGGATGGTCATACCACAAGATCGAAGATCGTTACCTTACCTCCAACTATCTTC
AAGCCATATCCATTATGGACAGGTAAACAAATCATCACAACAGTTTTGTTGAATGTCACC
CCTCCAGATATGCCTGGTATCAATTTAATTTCTAAAAATAAGATTAAAAACGAATATTGG
GGCAAGGGATCTCTGGAAAACGAAGTTCTCTTCAAAGACGGCGCATTGCTATGTGGTATT
TTAGATAAGTCACAATACGGTGCCTCTAAGTATGGTATTGTTCATTCATTGCATGAAGTT
TATGGCCCAGAAGTCGCTGCTAAAGTCTTATCCGTCTTAGGTAGACTATTTACCAACTAT
ATCACTGCCACGGCATTCACTTGTGGTATGGACGATTTACGTCTAACAGCAGAAGGTAAT
AAATGGAGGACTGACATCTTGAAGACGTCTGTTGATACAGGTCGTGAAGCCGCTGCAGAA
GTTACTAATTTAGATAAAGACACGCCAGCAGATGACCCAGAGTTATTAAAGAGGCTACAA
GAAATACTGCGTGATAATAATAAGTCAGGTATTCTTGACGCTGTTACATCCTCCAAGGTC
AATGCTATTACTTCTCAAGTCGTCTCCAAATGTGTTCCAGATGGTACTATGAAAAAGTTT
CCATGCAATTCTATGCAAGCTATGGCACTTTCTGGTGCTAAAGGTTCTAACGTTAATGTT
TCTCAAATTATGTGTTTGTTGGGTCAACAAGCTTTAGAAGGTAGAAGAGTGCCGGTAATG
GTTAGTGGTAAAACTCTGCCTTCTTTCAAACCTTATGAAACAGATGCCATGGCTGGTGGT
TATGTTAAGGGTCGTTTCTACTCTGGTATAAAGCCACAGGAGTATTACTTCCATTGTATG
GCTGGTCGTGAAGGTTTAATTGATACGGCCGTTAAAACATCTAGATCCGGTTATTTGCAA
CGTTGTCTAACAAAACAATTAGAAGGTGTTCACGTATCATACGATAACAGTATAAGAGAT
GCAGATGGTACGTTAGTGCAATTCATGTATGGTGGTGATGCCATCGATATAACCAAAGAA
TCACACATGACTCAATTCGAGTTCTGTTTAGACAATTACTACGCATTATTGAAGAAATAC
AACCCATCAGCACTGATTGAACATTTGGATGTTGAATCAGCTTTGAAGTACTCTAAAAAG
ACTTTGAAATACAGAAAGAAGCATAGCAAGGAGCCTCATTACAAACAGTCTGTAAAATAT
GATCCTGTTTTAGCTAAGTATAATCCTGCCAAATACCTGGGTTCCGTTTCTGAGAACTTC
CAAGATAAACTAGAATCTTTCTTAGACAAGAACTCTAAATTGTTCAAATCATCGGATGGT
GTTAACGAAAAGAAGTTCAGGGCCTTGATGCAATTGAAGTATATGCGTTCTCTAATTAAT
CCAGGTGAAGCTGTTGGTATTATTGCTTCACAATCTGTCGGTGAACCCTCAACACAGATG
ACATTGAATACCTTCCACTTTGCTGGTCACGGTGCCGCTAATGTGACACTAGGTATTCCT
CGTTTAAGAGAAATTGTTATGACTGCCTCTGCTGCCATTAAAACTCCGCAAATGACTTTG
CCTATCTGGAATGATGTTTCTGATGAGCAAGCTGACACTTTCTGTAAGTCTATCTCAAAA
GTCCTATTATCCGAAGTGATAGATAAAGTGATAGTCACTGAAACTACCGGTACTTCAAAT
ACCGCTGGTGGAAATGCAGCTCGTTCGTATGTTATCCATATGAGATTCTTCGACAATAAT
GAATACAGTGAAGAATACGATGTTTCTAAAGAAGAATTACAAAACGTCATATCTAATCAA
TTCATACACTTGCTAGAAGCTGCTATTGTAAAAGAAATCAAAAAACAAAAGAGAACTACA
GGACCAGACATTGGTGTTGCAGTACCAAGGCTACAGACAGATGTTGCAAATAGTTCTTCG
AACTCTAAAAGGTTGGAAGAGGATAATGATGAAGAACAAAGTCATAAGAAAACTAAACAA
GCGGTTTCGTATGACGAGCCAGATGAAGATGAAATTGAAACTATGAGAGAAGCTGAAAAG
TCTTCTGACGAAGAAGGTATCGATTCCGATAAAGAAAGTGATTCTGACTCTGAGGACGAA
GACGTTGACATGAATGAACAAATTAATAAGAGTATTGTGGAAGCTAACAATAATATGAAC
AAAGTGCAGCGTGATCGTCAATCAGCGATTATTTCTCATCATAGATTCATCACCAAATAC
AATTTCGATGATGAATCTGGTAAATGGTGTGAATTTAAGCTAGAATTAGCAGCAGACACA
GAAAAGTTGTTGATGGTTAATATTGTTGAAGAAATCTGTAGGAAGTCTATCATTAGACAG
ATTCCTCATATTGACCGTTGTGTTCATCCAGAACCTGAAAACGGAAAACGTGTCCTTGTC
ACAGAAGGTGTTAATTTCCAAGCTATGTGGGATCAAGAGGCATTTATCGACGTTGATGGT
ATTACATCTAATGATGTTGCTGCTGTGTTGAAAACGTACGGTGTAGAAGCTGCTAGAAAT
ACTATTGTGAATGAAATTAATAACGTCTTCTCTCGTTATGCTATTTCTGTTTCTTTCCGT
CATTTAGACCTTATTGCTGACATGATGACCAGGCAAGGTACTTACTTAGCCTTTAACAGA
CAGGGTATGGAAACATCCACTTCATCTTTCATGAAGATGTCCTATGAAACTACATGTCAA
TTCTTGACCAAGGCTGTTCTAGATAATGAGCGTGAACAATTGGACAGTCCTTCCGCTAGA
ATTGTGGTGGGTAAATTGAACAATGTTGGTACGGGTTCATTTGATGTGTTAGCAAAGGTT
CCAAATGCGGCTTAG
Protein sequence predicted based on nucleotide sequence
MDISKPVGSEITSVDFGILTAKEIRNLSAKQITNPTVLDNLGHPVSGGLYDLALGAFLRN LCSTCGLDEKFCPGHQGHIELPVPCYNPLFFNQLYIYLRASCLFCHHFRLKSVEVHRYAC KLRLLQYGLIDESYKLDEITLGSLNSSMYTDDEAIEDNEDEMDGEGSKQSKDISSTLLNE LKSKRSEYVDMAIAKALSDGRTTERGSFTATVNDERKKLVHEFHKKLLSRGKCDNCGMFS PKFRKDGFTKIFETALNEKQITNNRVKGFIRQDMIKKQKQAKKLDGSNEASANDEESFDV GRNPTTRPKTGSTYILSTEVKNILDTVFRKEQCVLQYVFHSRPNLSRKLVKADSFFMDVL VVPPTRFRLPSKLGEEVHENSQNQLLSKVLTTSLLIRDLNDDLSKLQKDKVSLEDRRVIF SRLMNAFVTIQNDVNAFIDSTKAQGRTSGKVPIPGVKQALEKKEGLFRKHMMGKRVNYAA RSVISPDPNIETNEIGVPPVFAVKLTYPEPVTAYNIAELRQAVINGPDKWPGATQIQNED GSLVSLIGMSVEQRKALANQLLTPSSNVSTHTLNKKVYRHIKNRDVVLMNRQPTLHKASM MGHKVRVLPNEKTLRLHYANTGAYNADFDGDEMNMHFPQNENARAEALNLANTDSQYLTP TSGSPVRGLIQDHISAGVWLTSKDSFFTREQYQQYIYGCIRPEDGHTTRSKIVTLPPTIF KPYPLWTGKQIITTVLLNVTPPDMPGINLISKNKIKNEYWGKGSLENEVLFKDGALLCGI LDKSQYGASKYGIVHSLHEVYGPEVAAKVLSVLGRLFTNYITATAFTCGMDDLRLTAEGN KWRTDILKTSVDTGREAAAEVTNLDKDTPADDPELLKRLQEILRDNNKSGILDAVTSSKV NAITSQVVSKCVPDGTMKKFPCNSMQAMALSGAKGSNVNVSQIMCLLGQQALEGRRVPVM VSGKTLPSFKPYETDAMAGGYVKGRFYSGIKPQEYYFHCMAGREGLIDTAVKTSRSGYLQ RCLTKQLEGVHVSYDNSIRDADGTLVQFMYGGDAIDITKESHMTQFEFCLDNYYALLKKY NPSALIEHLDVESALKYSKKTLKYRKKHSKEPHYKQSVKYDPVLAKYNPAKYLGSVSENF QDKLESFLDKNSKLFKSSDGVNEKKFRALMQLKYMRSLINPGEAVGIIASQSVGEPSTQM TLNTFHFAGHGAANVTLGIPRLREIVMTASAAIKTPQMTLPIWNDVSDEQADTFCKSISK VLLSEVIDKVIVTETTGTSNTAGGNAARSYVIHMRFFDNNEYSEEYDVSKEELQNVISNQ FIHLLEAAIVKEIKKQKRTTGPDIGVAVPRLQTDVANSSSNSKRLEEDNDEEQSHKKTKQ AVSYDEPDEDEIETMREAEKSSDEEGIDSDKESDSDSEDEDVDMNEQINKSIVEANNNMN KVQRDRQSAIISHHRFITKYNFDDESGKWCEFKLELAADTEKLLMVNIVEEICRKSIIRQ IPHIDRCVHPEPENGKRVLVTEGVNFQAMWDQEAFIDVDGITSNDVAAVLKTYGVEAARN TIVNEINNVFSRYAISVSFRHLDLIADMMTRQGTYLAFNRQGMETSTSSFMKMSYETTCQ FLTKAVLDNEREQLDSPSARIVVGKLNNVGTGSFDVLAKVPNAA*
Coding Sequence Details
Insert Sequence Verified?:
N
Verification Method:
Insert was fully sequenced in parent vector
Reference Sequence Annotations:
Start on reference sequence
1
End on reference sequence
4995
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
yeast
Marker:
URA
Host Type: bacterial Marker: ampicillin
Host Type: bacterial Marker: ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pBY011
Synonyms:
''
Description:
with tag/fusion/marker assay, Gateway®, acceptor (destination), recombinational cloning clone, inducible expression, yeast expression, yeast shuttle expression with ARAC5R and Ura3 promoter and AmpR promoter and bla and ori and CAP binding site and lac promoter and lac operator and M13 reverse and attR2 and CmR and lac UV5 promoter and attR1 and GAL1, 10 and Ura3 and UAS and M13 forward; 50ug/mL of Kanamycin resistance in Pichia;
Comments:
This is a bacteria-yeast shuttle vector useful for expressing cDNAs in yeast that includes an auxotrophic marker for selection in yeast.
Size (bp):
8121
Parent Vector:
Name:
YCplac33
Description: Parent vector is a CEN4, URA3, ampR, ColE1 vector from R.D. Geitz described in Gene 74(2) 5270534 (1988). The Gal1-10 promoter and the Gateway-type attR recombination cassette (reference frame A, see information at the Invitrogen website) were inserted into the parent to create pBY011.
Properties:
Gateway, acceptor (destination), inducible expression, recombinational cloning, with tag/fusion/marker, yeast expression, yeast shuttle
Author Name:
Bhullar
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
CmR
confers resistance to chloramphenicol
5563
6222
CDS
Ura3
yeast auxotrophic marker, counterselectable with 5-fluoroorotic acid (5-FOA)
1460
2263
CDS
bla
allows cytosolic expression of beta-lactamase
2768
3556
primer_bind
M13 forward
common sequencing primer, one of multiple similar variants
7183
7199
primer_bind
M13 reverse
common sequencing primer, one of multiple similar variants
4702
4718
promoter
AmpR promoter
null
2590
2694
promoter
GAL1, 10
divergent inducible promoter, regulated by Gal4
6481
7145
promoter
Ura3 promoter
S. cerevisiae URA3
2264
2479
promoter
lac promoter
promoter for the E. coli lac operon
4640
4670
promoter
lac UV5 promoter
E. coli lac promoter with an "up" mutation
6276
6306
protein binding site
attR1
recombination site for the Gateway LR reaction
6331
6455
protein binding site
attR2
recombination site for the Gateway LR reaction
4747
4871
protein binding site
CAP binding site
CAP binding activates transcription in the presence of cAMP.
4604
4625
protein binding site
lac operator
The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG).
4678
4694
protein binding site
UAS
upstream activating sequence mediating Gal4-dependent induction
6812
6929
rep_origin
ARAC5R
S. cerevisiae autonomously replicating sequence ARS1/ARS416
288
1125
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
3727
4315
