RIA1 (S. cerevisiae ) in pBY011 (yeast expression vector)

Explanation of Terms

Gene:
Gene Symbol: 
Gene Name: 
Original Clone ID:
FLH260879.01X
Keyword:
None
Species:
Saccharomyces cerevisiae
Type:
cDNA
Vector Name:
pBY011
Format: 
CLOSED
Source:
HIP
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
ScCD00097077
Price: 
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 3333nts
Open reading frame: 1 to 3333

ATGCCTAGAGTGGAATCGGAGACTTACAAACGTCTGCAAAATGACCCTTCCTGTATAAGA
AATATATGTATTGTTGCTCATGTTGATCACGGTAAAACTTCACTTTCAGATTCTCTTTTG
GCTTCGAATGGTATTATATCACAAAGGCTTGCCGGCAAAATTCGATTCTTAGATGCCAGG
CCTGACGAACAACTACGTGGTATCACAATGGAATCATCGGCTATCTCTTTATATTTTAGG
GTATTGCGTAAACAAGAAGGTTCCGATGAGCCTCTTGTAAGCGAGCATCTAGTAAACTTA
ATTGATTCCCCAGGTCACATTGATTTTTCAAGTGAGGTCAGTGCAGCTTCAAGACTATGT
GATGGTGCTGTCGTACTGGTTGATGTCGTAGAGGGGGTATGCTCGCAAACCGTCACAGTG
TTGAGACAATGTTGGACGGAGAAGCTGAAGCCCATTTTAGTATTGAATAAGATTGATAGA
TTGATCACAGAACTACAACTAACTCCCCAAGAAGCTTATATTCACTTGTCCAAAGTCATT
GAACAAGTCAACTCAGTTATCGGGTCCTTTTTCGCAAACGAGAGACAATTGGATGATTTA
TTTTGGAGGGAGCAATTAGAAAAGAACGAAAACGCGGAATATATTGAAAAGGATGATTCG
GGTATTTACTTTAACCCTACTGATAACAACGTTATTTTTGCTTCCGCAATTGATGGTTGG
GGTTTCAACATCGGTCAATTGGCCAAATTTTACGAACAAAAATTAGGCGCTAAAAGGGAA
AACCTACAGAAAGTTTTATGGGGTGATTTCTATATGGATCCCAAGACAAAAAAAATTATT
AACAATAAAGGCCTTAAGGGAAGATCCCTGAAACCCCTTTTCACATCGCTAATCCTCGAA
AATATATGGAAAATTTATCAAAACATAATCACATCGAGAGATTCTGAAATGGTCGAAAAA
ATAGCAAAAACTTTAAATATTAAGCTACTTGCACGTGATCTAAGGTCGAAGGACGATAAA
CAATTGTTAAGAACTATCATGGGTCAATGGCTACCCGTCAGCACAGCAGTATTGTTAACA
GTTATTGAAAAATTACCTTCACCTTTGGAATCACAAACCGATCGTTTGAACACTATTTTG
GTCTCCGAATCCGACACAGCAGCCATGGATCCAAGACTTCTGAAAGCCATGAAGACATGT
GATAAAGAAGGACCAGTTAGTGCATACGTATCTAAGATGCTTTCAATTCCTAGAGAAGAA
TTACCTGTTGAATCTAAGAGGATAGCATCTTCGGATGAGCTGATGGAGAGAAGTAGAAAG
GCACGTGAGGAAGCACTTAATGCTGCAAAACATGCTGGTATGGTAGAAAATATGGCAATG
ATGGATTTGAATGACAATTCTAAAAATACATCAGATTTATATAAAAGAGCAAAAGATACC
GTTATGACCCCCGAAGTAGGAGAACAGACAAAGCCAAAACCTTCAAGAAATAATGATGTT
TTCTGTGTTGTTTCAGAGCCTTCTTCCGCCTTGGATTTAGAATTTGAATATGAGGGGGAG
GATGATTCAGATAGTCAAGATAACTTCGGCCTAGATTTTGTACCAACTGACATAGATCCA
AATGACCCTCTAAGTTCAATGTTTGAATATGAGGAAGAAGATCCACTTTTGGAGAGTATT
AAGCAAATTTCAGAGGATGTGAACGATGAAGTAGATGATATTTTTGACGAAAAGGAAGAA
TGCTTGGTAGCATTTGCAAGAATATACAGTGGCACACTAAGAGTTGGTCAGGAGATTTCG
GTATTGGGTCCAAAATATGATCCAAAATGTCCAGAGGAGCATATTGAAACTGCGATTATT
ACGCATTTATATTTGTTTATGGGTAAGGAATTGGTTCCTTTGGACGTTTGTCCATCAGGA
AATATAGTCGGTATTCGTGGTCTGGCCGGTAAAGTTTTAAAGAGTGGTACGTTGATAGAG
AAGGGCGTACAAGGCGTCAATCTAGCGGGCGTCAATTTCCATTTTACGCCAATCGTTCGT
GTTGCTGTGGAGCCAGCCAATCCTGTGGAAATGAGCAAGTTGGTACGAGGATTAAAGTTG
TTGGATCAAGCAGATCCTTGCGTGCACACATACGTTGAAAATACTGGTGAACACATTCTG
TGTACGGCCGGTGAATTACATTTAGAAAGATGCCTAAAAGATTTAACAGAGAGATTTGCA
GGAATAGAAATTACACATTCTGAACCGGCTATTCCATATAGGGAAACATTTTTATCAGCC
TCAGATATGAATCCACCGCAGAATTCACAGCTGGGAAGGGGTGTTCATGAACTACTATTG
AGCCAGTACAAGATTACATTCAGAACGTTCCCATTAAGCGGTAAAGTTACAGACTTTTTA
TCTCAACATCAAAATAGCATTAAAAACATTTTGAAGACGTCCACTTCAAGCATGGATCCC
GTCATAGAATCAACTGGAAGTTCTTTTCTAGACAAAAAGTCTTTACTCGTTGCTTTTGAA
GAGGTCATAAATCAGGAGGAGAAGAGCAGAGAACTATTAAGTGGGTTTAAAGTAAAGTTA
GCAGGTTTTGGGCCAAGTAGAGTTGGGTGCAACATTTTGCTGTCGCAGGATAATCTTCTT
GGAAGCCTATTTGAAGGTACTCCAGCAGCCTTTGAGTATTCAGATTCCATCAAAAATGGC
TTCCAACTGGCTGTTAGCGAAGGACCTCTGGCAAATGAGCCCGTACAGGGAATGTGTGTT
CTTGTTGAAAGCGTACATAAGATGTCACAAGACGAAATTGAATCTATTGAAGATCCGCGC
TACCAACAACATATTGTGGACTTATCAGGGAGGTTGATAACTTCTACAAGGGACGCTATA
CATGAGGCCTTTTTAGACTGGTCCCCAAGAATTATGTGGGCAATTTACTCATGTGATATT
CAAACCTCTGTCGATGTACTTGGAAAAGTGTATGCCGTTATTCTACAGAGGCATGGTAAA
ATCATTTCTGAAGAAATGAAAGAAGGTACACCGTTTTTTCAAATTGAGGCACATGTGCCC
GTTGTGGAAGCATTCGGTTTAAGTGAAGATATTCGTAAACGAACATCTGGTGCAGCACAA
CCTCAATTGGTATTTTCCGGATTCGAGTGTATCGATTTAGATCCATTTTGGGTCCCAACC
ACAGAAGAAGAATTGGAGGAACTAGGTGATACAGCAGATAGAGAGAATATTGCCCGTAAG
CATATGAATGCCATCAGAAGAAGGAAAGGTCTATTCATTGAAGAAAAAGTTGTCGAAAAT
GCCGAAAAACAACGTACTTTGAAAAAGAATTAG

Protein sequence predicted based on nucleotide sequence

MPRVESETYKRLQNDPSCIRNICIVAHVDHGKTSLSDSLLASNGIISQRLAGKIRFLDAR
PDEQLRGITMESSAISLYFRVLRKQEGSDEPLVSEHLVNLIDSPGHIDFSSEVSAASRLC
DGAVVLVDVVEGVCSQTVTVLRQCWTEKLKPILVLNKIDRLITELQLTPQEAYIHLSKVI
EQVNSVIGSFFANERQLDDLFWREQLEKNENAEYIEKDDSGIYFNPTDNNVIFASAIDGW
GFNIGQLAKFYEQKLGAKRENLQKVLWGDFYMDPKTKKIINNKGLKGRSLKPLFTSLILE
NIWKIYQNIITSRDSEMVEKIAKTLNIKLLARDLRSKDDKQLLRTIMGQWLPVSTAVLLT
VIEKLPSPLESQTDRLNTILVSESDTAAMDPRLLKAMKTCDKEGPVSAYVSKMLSIPREE
LPVESKRIASSDELMERSRKAREEALNAAKHAGMVENMAMMDLNDNSKNTSDLYKRAKDT
VMTPEVGEQTKPKPSRNNDVFCVVSEPSSALDLEFEYEGEDDSDSQDNFGLDFVPTDIDP
NDPLSSMFEYEEEDPLLESIKQISEDVNDEVDDIFDEKEECLVAFARIYSGTLRVGQEIS
VLGPKYDPKCPEEHIETAIITHLYLFMGKELVPLDVCPSGNIVGIRGLAGKVLKSGTLIE
KGVQGVNLAGVNFHFTPIVRVAVEPANPVEMSKLVRGLKLLDQADPCVHTYVENTGEHIL
CTAGELHLERCLKDLTERFAGIEITHSEPAIPYRETFLSASDMNPPQNSQLGRGVHELLL
SQYKITFRTFPLSGKVTDFLSQHQNSIKNILKTSTSSMDPVIESTGSSFLDKKSLLVAFE
EVINQEEKSRELLSGFKVKLAGFGPSRVGCNILLSQDNLLGSLFEGTPAAFEYSDSIKNG
FQLAVSEGPLANEPVQGMCVLVESVHKMSQDEIESIEDPRYQQHIVDLSGRLITSTRDAI
HEAFLDWSPRIMWAIYSCDIQTSVDVLGKVYAVILQRHGKIISEEMKEGTPFFQIEAHVP
VVEAFGLSEDIRKRTSGAAQPQLVFSGFECIDLDPFWVPTTEEELEELGDTADRENIARK
HMNAIRRRKGLFIEEKVVENAEKQRTLKKN*

Coding Sequence Details

Insert Sequence Verified?:
N
Verification Method:
Insert was fully sequenced in parent vector
Reference Sequence Annotations:
Start on reference sequence 1
End on reference sequence 3333
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  yeast    Marker:  URA
Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition: 100 ug/mL ampicillin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable