NTSR1 (Homo sapiens) in pDNR-Dual (Creator donor/master vector)

Explanation of Terms

Gene:
Gene Symbol:  NTSR1
Gene Name:  neurotensin receptor 1 (high affinity)
Original Clone ID:
FLH009151.01L
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDNR-Dual
Format: 
FUSION
Source:
HIP
Description:
None
Comments:
None
Mutation / Discrepancy:
No / Yes
Publications:
PMID: 16512675
Title: Functional proteomics approach to investigate the biological activities of cDNAs implicated in breast cancer.
Authors:
HIP
HsCD00000977
Price: 
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 1257nts
Open reading frame: 1 to 1257

ATGCGCCTCAACAGCTCCGCGCCGGGAACCCCGGGCACGCCGGCCGCCGACCCCTTCCAG
CGGGCGCAGGCCGGACTGGAGGAGGCGCTGCTGGCCCCGGGCTTCGGCAACGCTTCGGGC
AACGCGTCGGAGCGCGTCCTGGCGGCACCCAGCAGCGAGCTGGACGTGAACACCGACATC
TACTCCAAGGTGCTGGTGACCGCCGTGTACCTGGCGCTCTTCGTGGTGGGCACGGTGGGC
AACACGGTGACGGCGTTCACGCTGGCGCGGAAGAAGTCGCTGCAGAGCCTGCAGAGCACG
GTGCATTACCACCTGGGCAGCCTGGCGCTGTCCGACCTGCTCACCCTGCTGCTGGCCATG
CCCGTGGAGCTGTACAACTTCATCTGGGTGCACCACCCCTGGGCCTTCGGCGACGCCGGC
TGCCGCGGCTACTACTTCCTGCGCGACGCCTGCACCTACGCCACGGCCCTCAACGTGGCC
AGCCTGAGTGTGGAGCGCTACCTGGCCATCTGCCACCCCTTCAAGGCCAAGACCCTCATG
TCCCGAAGCCGCACCAAGAAGTTCATCAGCGCCATCTGGCTCGCCTCGGCCCTGCTGACG
GTGCCTATGCTGTTCACCATGGGCGAGCAGAACCGCAGCGCCGACGGCCAGCACGCCGGC
GGCCTGGTGTGCACCCCCACCATCCACACTGCCACCGTCAAGGTCGTCATACAGGTCAAC
ACCTTCATGTCCTTCATATTCCCCATGGTGGTCATCTCGGTCCTGAACACCATCATCGCC
AACAAGCTGACCGTCATGGTACGCCAGGCGGCCGAGCAGGGCCAAGTGTGCACGGTCGGG
GGCGAGCACAGCACATTCAGCATGGCCATCGAGCCTGGCAGGGTCCAGGCCCTGCGGCAC
GGCGTGCGCGTCCTACGTGCAGTGGTCATCGCCTTTGTGGTCTGCTGGCTGCCCTACCAC
GTGCGGCGCCTCATGTTCTGCTACATCTCGGATGAGCAGTGGACTCCGTTCCTCTATGAC
TTCTACCACTACTTCTACATGGTGACCAACGCACTCTTCTACGTCAGCTCCACCATCAAC
CCCATCCTGTACAACCTCGTCTCTGCCAACTTCCGCCACATCTTCCTGGCCACACTGGCC
TGCCTCTGCCCGGTGTGGCGGCGCAGGAGGAAGAGGCCAGCCTTCTCGAGGAAGGCCGAC
AGCGTGTCCAGCAACCACACCCTCTCCAGCAATGCCACCCGCGAGACGCTGTACTTG

Protein sequence predicted based on nucleotide sequence

MRLNSSAPGTPGTPAADPFQRAQAGLEEALLAPGFGNASGNASERVLAAPSSELDVNTDI
YSKVLVTAVYLALFVVGTVGNTVTAFTLARKKSLQSLQSTVHYHLGSLALSDLLTLLLAM
PVELYNFIWVHHPWAFGDAGCRGYYFLRDACTYATALNVASLSVERYLAICHPFKAKTLM
SRSRTKKFISAIWLASALLTVPMLFTMGEQNRSADGQHAGGLVCTPTIHTATVKVVIQVN
TFMSFIFPMVVISVLNTIIANKLTVMVRQAAEQGQVCTVGGEHSTFSMAIEPGRVQALRH
GVRVLRAVVIAFVVCWLPYHVRRLMFCYISDEQWTPFLYDFYHYFYMVTNALFYVSSTIN
PILYNLVSANFRHIFLATLACLCPVWRRRRKRPAFSRKADSVSSNHTLSSNATRETLYL

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
End on reference sequence 1629
Start on reference sequence 373
Mutations:
None
Discrepancy:
a189g
                                                    

5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition: 100 ug/mL ampicillin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable