Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
FLH463521.01X
Keyword:
None
Species:
Saccharomyces cerevisiae
Type:
cDNA
Vector Name:
ZM552
Format:
CLOSED
Source:
HIP
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
ScCD00098350
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 3456nts
Open reading frame: 1 to 3456
ATGGATCAGGATAACAACAACCACAATGACAGCAATAGGCTGCACCCACCTGATATACAT
CCAAATTTGGGCCCTCAATTGTGGCTGAATAGTAGCGGTGATTTTGACGACAACAACAAC
AACAACAACAACAACAACAATAATAATAGCACAAGACCACAAATGCCATCACGAACTAGA
GAAACGGCAACTTCGGAAAGAAATGCAAGTGAGGTTAGGGATGCAACGCTAAATAATATC
TTTAGGTTCGATAGTATTCAACGGGAAACGCTTTTGCCAACCAACAACGGACAACCGCTA
AATCAAAACTTTTCGCTGACATTTCAACCACAACAGCAAACAAATGCGCTGAACGGGATT
GACATAAACACTGTGAACACAAACCTTATGAATGGTGTCAATGTTCAAATAGATCAACTT
AATCGATTGTTACCGAACCTACCAGAGGAAGAACGGAAGCAAATCCACGAATTCAAGCTA
ATAGTGGGCAAAAAAATCCAAGAGTTTCTGGTTGTTATAGAGAAACGTAGAAAAAAAATA
CTGAACGAAATTGAGCTAGACAACCTTAAACTAAAGGAGCTACGTATTGATAACTCCCCA
CAAGCAATTAGTTATTTGCATAAATTACAAAGAATGAGGCTTAGGGCGCTAGAGACAGAA
AACATGGAAATTAGAAATTTAAGGCTAAAAATATTAACAATTATAGAAGAGTACAAAAAG
TCATTATATGCATACTGCCATTCCAAGCTAAGAGGTCAACAAGTGGAAAATCCAACAGAT
AATTTCATCATTTGGATAAACTCCATAGATACTACTGAATCATCTGACTTGAAAGAAGGG
CTACAAGATCTTTCGAGATATTCAAGGCAGTTCATAAATAATGTGCTTTCGAATCCATCA
AATCAAAACATATGTACGAGTGTCACCCGAAGATCACCTGTGTTTGCCCTAAACATGCTA
CCCTCGGAAATATTACACTTAATATTAGATAAACTTAACCAAAAATATGATATTGTAAAA
TTCCTTACCGTTTCCAAACTCTGGGCTGAAATAATTGTGAAGATACTTTATTACAGACCG
CACATCAACAAAAAGAGTCAATTAGACTTGTTTTTAAGGACTATGAAGTTAACTTCTGAA
GAAACTGTATTCAACTATCGTTTAATGATCAAAAGATTAAATTTTTCATTTGTTGGTGAC
TACATGCACGATACAGAGCTTAACTATTTTGTCGGATGTAAGAATTTGGAGCGACTAACT
TTAGTATTTTGCAAGCATATAACCAGTGTTCCAATATCGGCTGTTTTGAGAGGATGTAAA
TTTCTCCAAAGTGTGGATATCACTGGAATAAGAGACGTTTCCGATGACGTATTTGATACC
TTAGCGACATATTGTCCCAGAGTACAGGGCTTTTATGTTCCTCAGGCAAGGAATGTAACA
TTCGATTCACTGCGGAATTTCATAGTCCATTCCCCGATGTTGAAAAGAATAAAAATCACA
GCAAACAATAACATGAATGACGAATTAGTAGAACTATTAGCCAACAAATGCCCTTTGCTT
GTAGAGGTCGATATAACATTAAGTCCAAATGTCACTGATTCTAGTTTGTTAAAACTCCTC
ACTAGGTTAGTTCAGCTGAGGGAATTCAGAATAACTCATAATACGAATATTACGGATAAT
CTTTTCCAGGAGCTTTCTAAAGTAGTTGACGATATGCCCTCTTTAAGATTGATTGATCTT
TCTGGATGTGAAAATATTACAGATAAAACTATAGAAAGTATAGTCAATTTAGCCCCTAAA
TTACGTAATGTTTTTCTAGGCAAGTGTAGCCGAATTACAGATGCATCGTTGTTCCAATTA
TCGAAGCTGGGCAAAAACTTGCAAACAGTGCATTTTGGGCACTGTTTCAATATAACTGAT
AACGGGGTAAGAGCACTCTTTCATTCATGTACAAGAATACAGTATGTGGACTTTGCGTGC
TGTACGAATTTAACCAATAGAACTCTTTATGAACTAGCAGACTTACCAAAATTAAAGAGA
ATTGGCCTTGTCAAATGTACGCAAATGACTGACGAGGGTTTGTTGAATATGGTTTCCTTG
CGAGGCCGAAATGATACTTTAGAAAGGGTACATTTATCTTACTGTTCTAATTTAACAATA
TATCCGATATATGAGCTTCTAATGTCTTGCCCAAGGCTCTCACATTTGTCTTTGACTGCT
GTTCCGTCATTTTTACGCCCCGATATAACGATGTATTGCAGGCCTGCACCCTCAGACTTT
AGTGAAAATCAACGTCAAATATTCTGCGTATTTTCAGGGAAAGGTGTTCATAAACTTCGC
CATTATTTAGTAAATTTAACGTCGCCCGCTTTTGGACCACATGTCGATGTAAATGATGTT
TTGACAAAATATATTAGATCCAAGAATTTGATATTTAACGGTGAAACACTTGAAGATGCT
CTTAGGAGAATCATAACTGATTTAAATCAAGATTCCGCTGCAATTATAGCTGCTACAGGA
TTAAATCAAATCAACGGTCTAAATAACGATTTTCTTTTCCAGAATATCAATTTTGAACGA
ATAGATGAAGTATTCAGTTGGTATCTCAATACTTTTGATGGCATTAGGATGAGCTCGGAG
GAAGTTAACTCACTATTATTGCAAGTAAACAAGACGTTTTGTGAAGATCCATTTAGTGAT
GTGGACGATGATCAAGATTATGTCGTAGCACCTGGTGTAAACCGGGAAATTAACAGTGAA
ATGTGTCATATTGTTAGAAAATTCCATGAGTTAAATGATCATATTGATGATTTCGAGGTG
AATGTTGCTAGTTTGGTAAGAGTTCAGTTTCAGTTTACTGGTTTTTTACTTCATGAAATG
ACTCAAACCTATATGCAAATGATTGAATTAAACAGACAAATTTGTTTAGTACAAAAAACG
GTTCAGGAATCGGGCAACATAGATTACCAAAAAGGGCTTTTAATATGGCGACTTTTATTC
ATTGACAAATTCATTATGGTGGTTCAGAAGTACAAGCTCTCCACCGTTGTTTTGAGACTA
TATTTAAAAGATAACATAACATTGTTAACCAGACAAAGAGAACTATTAATAGCCCACCAA
AGATCAGCATGGAATAACAATAATGACAATGACGCCAACCGGAACGCCAACAACATAGTG
AATATTGTATCGGATGCTGGGGCAAACGATACAAGTAACAATGAAACTAACAATGGTAAT
GATGACAATGAAACAGAAAATCCAAATTTCTGGCGTCAGTTTGGCAATAGAATGCAAATA
TCACCTGACCAGATGAGGAATCTCCAAATGGGACTTCGTAATCAGAACATGGTTAGGAAC
AATAACAACAACACAATTGACGAATCAATGCCTGACACTGCCATTGATTCTCAAATGGAT
GAAGCATCAGGAACGCCCGATGAAGATATGTTATAG
Protein sequence predicted based on nucleotide sequence
MDQDNNNHNDSNRLHPPDIHPNLGPQLWLNSSGDFDDNNNNNNNNNNNNSTRPQMPSRTR ETATSERNASEVRDATLNNIFRFDSIQRETLLPTNNGQPLNQNFSLTFQPQQQTNALNGI DINTVNTNLMNGVNVQIDQLNRLLPNLPEEERKQIHEFKLIVGKKIQEFLVVIEKRRKKI LNEIELDNLKLKELRIDNSPQAISYLHKLQRMRLRALETENMEIRNLRLKILTIIEEYKK SLYAYCHSKLRGQQVENPTDNFIIWINSIDTTESSDLKEGLQDLSRYSRQFINNVLSNPS NQNICTSVTRRSPVFALNMLPSEILHLILDKLNQKYDIVKFLTVSKLWAEIIVKILYYRP HINKKSQLDLFLRTMKLTSEETVFNYRLMIKRLNFSFVGDYMHDTELNYFVGCKNLERLT LVFCKHITSVPISAVLRGCKFLQSVDITGIRDVSDDVFDTLATYCPRVQGFYVPQARNVT FDSLRNFIVHSPMLKRIKITANNNMNDELVELLANKCPLLVEVDITLSPNVTDSSLLKLL TRLVQLREFRITHNTNITDNLFQELSKVVDDMPSLRLIDLSGCENITDKTIESIVNLAPK LRNVFLGKCSRITDASLFQLSKLGKNLQTVHFGHCFNITDNGVRALFHSCTRIQYVDFAC CTNLTNRTLYELADLPKLKRIGLVKCTQMTDEGLLNMVSLRGRNDTLERVHLSYCSNLTI YPIYELLMSCPRLSHLSLTAVPSFLRPDITMYCRPAPSDFSENQRQIFCVFSGKGVHKLR HYLVNLTSPAFGPHVDVNDVLTKYIRSKNLIFNGETLEDALRRIITDLNQDSAAIIAATG LNQINGLNNDFLFQNINFERIDEVFSWYLNTFDGIRMSSEEVNSLLLQVNKTFCEDPFSD VDDDQDYVVAPGVNREINSEMCHIVRKFHELNDHIDDFEVNVASLVRVQFQFTGFLLHEM TQTYMQMIELNRQICLVQKTVQESGNIDYQKGLLIWRLLFIDKFIMVVQKYKLSTVVLRL YLKDNITLLTRQRELLIAHQRSAWNNNNDNDANRNANNIVNIVSDAGANDTSNNETNNGN DDNETENPNFWRQFGNRMQISPDQMRNLQMGLRNQNMVRNNNNNTIDESMPDTAIDSQMD EASGTPDEDML*
Coding Sequence Details
Insert Sequence Verified?:
N
Verification Method:
Insert was fully sequenced in parent vector
Reference Sequence Annotations:
Start on reference sequence
1
End on reference sequence
3456
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
yeast
Marker:
LEU2
Host Type: bacterial Marker: ampicillin
Host Type: bacterial Marker: ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
ZM552
Synonyms:
ZM552
Description:
Yeast expression vector with N-terminal 3X V5 epitope tag; ampicillin resistance in bacteria; LEU2 resistance in yeast; Gateway cloning.
Comments:
ZM511 Stu1 vector plus Gateway attR frame B cassette EcoRV fragment; same as ZM515. Cloning into the vector removes the ccdB cassette and chloramphenicol resistance.
Size (bp):
8302
Parent Vector:
None
Properties:
Gateway, acceptor (destination), recombinational cloning, with tag/fusion/marker, yeast expression
Author Name:
Zarmik Moqtaderi
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
primer site
M13F
M13 forward sequencing primer
8286
8301
primer site
M13R
M13 reverse sequencing primer
2630
2647
promoter
CMD1
CMD1 promoter
295
484
recombination site
attR1
attR1 recombinational cloning site
650
666
recombination site
attR2
attR2 recombination site
2337
2352
reporter gene
LacZ
LacZ alpha gene
8146
8214
selectable marker
AmpR
ampcillin resistance
3795
4454
selectable marker
CmR
chloramphenicol resistance. removed with the ccdB cassette in clones with insert
883
1539
selectable marker
LEU2
LEU2 selectable marker in yeast
5273
5344
tag
V5
N-terminal triple V5 tag
494
631
trxn termination sequence
CYC1
CYC1 terminator
2377
2582
yeast ARS
ARS
ARS yeast origin of replication
0
0
yeast centromere
CEN
yeast centromere
0
0
