Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
FLH464163.01X
Keyword:
None
Species:
Saccharomyces cerevisiae
Type:
cDNA
Vector Name:
ZM552
Format:
CLOSED
Source:
HIP
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
ScCD00098648
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 7107nts
Open reading frame: 1 to 7107
ATGGAATCACACGTCAAATATCTTGACGAATTGATATTGGCAATAAAAGACCTGAACTCG
GGGGTGGATTCAAAGGTGCAGATTAAAAAAGTGCCCACGGATCCATCTTCTTCTCAGGAG
TACGCCAAGAGTTTAAAGATCCTGAACACCCTCATAAGAAACCTAAAAGATCAAAGAAGG
AACAATATCATGAAAAATGATACTATATTTTCGAAAACAGTTTCCGCCCTTGCCTTATTG
TTGGAGTACAACCCCTTCTTGCTTGTTATGAAGGATTCCAACGGGAACTTTGAGATACAA
AGGCTGATAGATGATTTCCTCAACATATCCGTTCTGAACTATGATAATTACCACAGAATA
TGGTTTATGAGGCGAAAATTAGGCAGCTGGTGCAAAGCATGTGTCGAATTTTACGGAAAA
CCTGCTAAGTTTCAGCTTACTGCACATTTTGAGAACACCATGAATCTTTACGAACAGGCC
TTGACTGAAGTCTTGTTGGGCAAGACTGAGCTTCTCAAATTTTATGACACCTTGAAGGGT
CTATACATTCTTTTATACTGGTTCACTTCGGAGTATAGTACTTTTGGGAACTCTATAGCA
TTCTTAGATTCTTCTTTGGGGTTCACGAAATTTGACTTTAACTTCCAACGATTAATCAGG
ATTGTTCTTTACGTCTTTGATTCCTGCGAACTAGCAGCACTAGAATATGCCGAAATCCAA
CTCAAATATATTTCTCTAGTTGTGGACTATGTTTGCAATAGAACAATTTCCACAGCCCTG
GATGCCCCAGCGTTAGTTTGTTGTGAACAATTAAAGTTTGTATTGACTACTATGCATCAT
TTTTTGGATAACAAGTATGGGCTCTTGGATAATGACCCCACTATGGCCAAAGGAATTCTT
CGACTATATTCTCTTTGCATTTCTAACGATTTCTCAAAATGCTTTGTAGACCACTTCCCA
ATTGACCAGTGGGCAGATTTTTCACAAAGTGAACATTTTCCGTTCACGCAGTTGACTAAT
AAAGCTCTCTCGATTGTATATTTTGATTTGAAAAGAAGGTCCCTACCTGTTGAAGCTTTA
AAGTACGATAATAAGTTCAACATCTGGGTATACCAATCGGAGCCGGACTCGAGCTTGAAA
AATGTCACTTCTCCCTTTGATGATCGATATAAGCAGCTGGAAAAGCTAAGGTTGCTAGTA
CTAAAGAAGTTTAACAAGACAGAAAGAGGAACTTTGCTCAAATACCGCGTGAACCAGCTA
AGTCCTGGATTTTTTCAAAGAGCTGGAAACGATTTCAAGCTAATTTTAAATGAAGCATCT
GTATCCATTCAAACTTGTTTCAAGACAAACAATATAACAAGGCTAACATCATGGACTGTA
ATTCTCGGACGTCTAGCCTGTCTAGAATCAGAGAAGTTTTCCGGCACTCTGCCAAATTCC
ACAAAGGATATGGATAATTGGTATGTTTGTCATTTATGCGATATTGAGAAAACTGGCAAC
CCTTTCGTGCGAATAAATCCAAATAGACCAGAGGCTGCGGGTAAATCAGAAATCTTCAGG
ATACTTCATTCAAACTTTCTATCTCACCCAAATATAGATGAATTTAGCGAATCTTTGTTA
AGTGGCATCTTATTTTCTCTACATAGGATATTTTCACACTTTCAACCTCCAAAACTTACA
GATGGAAACGGTCAAATCAATAAGAGCTTTAAACTGGTACAAAAGTGCTTTATGAATTCT
AACAGATACCTACGTTTATTAAGTACTAGAATTATACCTTTATTCAATATATCAGACTCT
CATAATTCCGAAGATGAACACACTGCCACGCTGATAAAGTTTCTACAATCTCAAAAATTG
CCAGTGGTGAAAGAAAATTTAGTCATTGCTTGGACACAATTAACATTGACGACTTCTAAT
GATGTATTTGATACACTACTTTTGAAACTGATTGATATTTTCAATTCTGATGATTATAGT
TTACGAATAATGATGACTTTGCAAATTAAAAATATGGCCAAAATTTTAAAGAAAACACCA
TATCAATTACTATCGCCTATTTTACCTGTATTACTAAGACAGTTGGGAAAAAACCTCGTG
GAAAGAAAAGTTGGCTTTCAAAATTTAATAGAATTATTGGGATATTCTTCAAAAACAATT
CTCGATATTTTCCAGAGATATATCATCCCTTATGCAATTATTCAATATAAGAGCGATGTG
CTAAGTGAAATTGCTAAGATTATGTGTGATGGCGATACAAGTTTAATTAACCAAATGAAG
GTTAATTTACTGAAAAAAAACAGTAGGCAAATATTTGCCGTAGCTTTGGTAAAACACGGA
TTATTTTCTTTGGATATCTTGGAAACCCTTTTTTTAAATAGGGCTCCAACTTTTGACAAA
GGATATATAACTGCATACCTTCCCGATTATAAAACTTTAGCTGAAATAACGAAGCTCTAC
AAAAACAGCGTTACTAAAGATGCAAGTGACAGCGAGAATGCTAATATGATTTTATGCTCT
TTGCGATTTTTAATCACCAATTTTGAAAAAGACAAAAGGCATGGTTCGAAGTACAAAAAT
ATCAATAACTGGACGGATGATCAGGAACAAGCGTTCCAAAAGAAACTACAGGATAATATC
TTAGGTATTTTCCAAGTTTTTTCGAGTGACATACATGATGTTGAAGGCCGCACCACTTAC
TACGAAAAGTTAAGGGTTATCAATGGCATTTCTTTTCTTATCATATATGCCCCCAAAAAA
TCAATAATTTCCGCATTAGCCCAGATTAGTATTTGTTTGCAAACAGGACTTGGGCTTAAG
GAAGTTCGATACGAGGCCTTTAGATGTTGGCATCTGTTAGTTCGCCATCTAAATGATGAA
GAACTCTCTACTGTTATAGATAGCTTAATTGCATTCATACTTCAAAAGTGGTCTGAGTTC
AACGGAAAACTTCGAAATATAGTGTACAGTATACTGGATACCTTAATCAAAGAGAAATCG
GACCTGATTTTGAAATTAAAACCTTACACTACTTTGGCTTTAGTAGGCAAGCCTGAATTA
GGTATTTTAGCTCGTGATGGCCAATTTGCAAGAATGGTGAATAAAATAAGAAGTACCACG
GACCTTATACCCATATTTGCTAATAACTTGAAAAGTAGTAACAAGTATGTCATAAACCAA
AATTTAGACGATATAGAGGTATATCTTCGGAGAAAGCAGACAGAAAGATCGATTGATTTT
ACACCAAAGAAGGTTGGGCAAACTTCTGATATAACATTAGTTTTGGGTGCTTTATTAGAC
ACTTCTCATAAGTTTAGAAATTTAGACAAGGACCTATGCGAGAAGTGCGCCAAATGTATC
AGTATGATTGGTGTTTTAGACGTTACAAAGCATGAGTTTAAAAGAACAACATATTCAGAA
AACGAAGTTTATGATTTGAATGATAGTGTTCAAACTATTAAGTTCTTGATATGGGTCATA
AATGATATCCTCGTTCCTGCGTTTTGGCAAAGTGAGAATCCCAGCAAGCAATTGTTTGTT
GCCCTTGTCATACAGGAATCATTAAAATATTGCGGGCTAAGTTCAGAGTCATGGGATATG
AACCATAAAGAATTATATCCAAATGAAGCCAAACTATGGGAAAAGTTTAACTCTGTCTCC
AAGACAACCATCTATCCGCTTTTATCTTCCTTGTATCTTGCGCAATCATGGAAAGAATAT
GTCCCGCTAAAATATCCTTCTAATAACTTCAAGGAAGGATACAAAATTTGGGTGAAAAGG
TTTACATTGGATTTATTGAAAACAGGTACAACAGAAAATCATCCATTACACGTGTTTTCC
TCTTTGATTAGGGAAGATGATGGCTCACTATCAAATTTTTTGCTACCTTATATTTCTCTG
GACATTATTATCAAGGCAGAAAAAGGAACTCCATACGCTGATATTTTAAACGGGATTATT
ATTGAATTTGACAGCATTTTCACGTGCAATCTGGAAGGAATGAATAACTTGCAAGTGGAT
TCGTTAAGAATGTGCTATGAATCCATCTTCAGAGTTTTCGAATATTGCAAAAAATGGGCA
ACTGAGTTTAAACAAAATTACAGTAAACTACACGGCACTTTTATCATTAAAGATACGAAA
ACAACTAACATGCTTTTGAGAATAGATGAGTTTTTGCGAACAACCCCTTCAGATTTGCTA
GCTCAACGCTCCTTAGAGACGGATTCTTTTGAAAGGTCTGCTCTATACCTTGAACAGTGC
TATCGACAGAATCCTCACGATAAGAACCAAAATGGACAACTACTGAAAAATTTACAAATC
ACATACGAAGAAATAGGAGACATTGACTCACTCGATGGTGTACTGAGAACCTTTGCTACA
GGAAACTTGGTTTCTAAAATTGAAGAATTGCAATATTCTGAAAACTGGAAACTCGCACAA
GACTGCTTTAATGTCCTCGGCAAATTTTCAGATGACCCCAAAACTACAACCAGGATGCTA
AAGTCTATGTATGACCACCAATTGTATTCTCAAATAATATCGAACTCTTCGTTCCATTCT
TCAGACGGAAAAATTTCTTTGTCTCCAGATGTGAAGGAATGGTACAGCATAGGTCTTGAA
GCTGCAAATCTAGAAGGCAATGTTCAAACTTTGAAAAATTGGGTAGAACAAATAGAGAGT
TTAAGAAATATTGACGATAGAGAAGTACTTTTGCAGTACAATATTGCGAAAGCTTTAATT
GCCATCTCAAACGAGGATCCATTAAGGACTCAAAAATACATCCACAATTCCTTTAGGCTT
ATCGGAACAAATTTTATAACGTCATCTAAAGAGACGACGCTGCTAAAGAAACAGAATTTA
TTGATGAAATTACACAGTTTATATGACCTCAGTTTTTTATCTTCTGCGAAAGATAAGTTT
GAATACAAAAGTAACACTACCATACTCGATTATCGAATGGAACGTATTGGGGCTGACTTC
GTGCCAAATCATTACATATTGTCAATGAGAAAGTCATTTGACCAATTGAAAATGAATGAA
CAAGCAGACGCTGACTTAGGAAAAACATTCTTCACTTTAGCCCAATTGGCGAGAAACAAC
GCTAGGCTAGATATAGCCTCCGAATCATTAATGCATTGTTTGGAAAGGCGGTTGCCTCAG
GCAGAGTTGGAGTTTGCTGAAATACTATGGAAGCAAGGTGAGAATGATAGAGCCTTAAAG
ATAGTGCAAGAAATACATGAAAAGTATCAAGAAAATTCCTCGGTTAATGCTCGCGATCGT
GCCGCCGTGCTATTAAAGTTTACTGAATGGTTAGACCTTTCGAACAATTCAGCGTCCGAA
CAAATTATTAAACAATATCAGGATATTTTTCAGATTGATTCTAAATGGGATAAACCATAT
TACTCTATTGGCTTATACTATAGTAGACTACTTGAGCGCAAAAAAGCAGAGGGTTATATT
ACTAATGGTCGTTTTGAGTACAGGGCAATATCTTACTTTTTATTGGCATTTGAAAAGAAC
ACTGCTAAAGTAAGAGAAAATTTGCCCAAAGTTATCACGTTTTGGCTAGATATTGCGGCC
GCATCAATTTCTGAAGCTCCTGGAAACAGAAAGGAAATGCTGAGTAAGGCTACGGAAGAT
ATATGTAGTCATGTTGAAGAAGCGCTGCAGCATTGTCCCACTTATATTTGGTACTTTGTT
TTGACTCAGTTGTTATCTAGGTTATTACATTCTCATCAATCATCGGCCCAGATAATAATG
CACATACTGCTAAGTTTGGCTGTTGAATACCCCTCTCATATTTTATGGTATATCACAGCC
CTTGTAAATTCCAATTCTTCAAAAAGAGTTCTTCGTGGTAAGCATATTTTAGAAAAGTAT
AGACAACATTCGCAAAATCCTCATGATCTAGTTTCTAGTGCATTGGATTTAACGAAAGCA
TTAACTCGTGTCTGTTTGCAAGATGTCAAAAGCATTACAAGTAGATCAGGCAAATCTTTA
GAAAAAGACTTCAAATTTGACATGAACGTGGCCCCATCTGCAATGGTTGTTCCAGTAAGA
AAAAATTTAGACATCATTTCACCACTAGAGTCTAACTCAATGAGGGGCTATCAACCATTT
AGGCCGGTTGTTTCTATAATTAGATTCGGATCATCTTATAAAGTGTTTTCTTCATTAAAG
AAGCCAAAACAATTGAACATAATAGGTTCAGATGGCAACATTTATGGGATCATGTGTAAG
AAGGAAGATGTCCGACAAGATAACCAATATATGCAGTTCGCCACAACAATGGATTTTCTT
CTGAGTAAGGACATAGCTTCAAGAAAAAGAAGCCTGGGCATAAATATTTACTCCGTACTA
TCTCTTCGAGAAGACTGTGGGATATTGGAAATGGTACCGAATGTTGTAACTTTAAGATCT
ATTCTTTCTACAAAGTACGAAAGTCTGAAAATTAAGTATAGCCTGAAAAGTCTACATGAT
AGGTGGCAGCACACCGCAGTAGATGGAAAACTCGAGTTTTACATGGAACAGGTAGATAAA
TTTCCTCCAATCTTGTACCAATGGTTTTTAGAAAACTTTCCTGATCCAATCAATTGGTTC
AACGCCAGGAATACGTATGCCAGATCTTACGCCGTCATGGCAATGGTTGGCCATATATTA
GGTCTAGGTGATAGGCACTGTGAAAACATATTACTAGATATACAGACGGGTAAAGTTCTT
CATGTAGACTTCGACTGTTTATTTGAGAAAGGCAAAAGGTTACCTGTCCCAGAAATTGTT
CCCTTCAGACTAACACCAAATTTATTGGATGCGTTGGGCATAATTGGGACAGAAGGAACA
TTTAAGAAGTCTAGTGAAGTCACGTTGGCTTTAATGAGAAAAAATGAAGTAGCGTTGATG
AATGTGATCGAAACAATTATGTACGATAGAAACATGGACCACTCAATTCAAAAAGCGCTA
AAGGTCTTAAGAAACAAAATCCGCGGTATAGATCCGCAGGATGGCCTGGTATTGAGTGTT
GCTGGCCAAACAGAAACATTGATCCAAGAAGCAACATCAGAAGACAATCTAAGCAAGATG
TATATTGGTTGGCTTCCATTTTGGTAG
Protein sequence predicted based on nucleotide sequence
MESHVKYLDELILAIKDLNSGVDSKVQIKKVPTDPSSSQEYAKSLKILNTLIRNLKDQRR NNIMKNDTIFSKTVSALALLLEYNPFLLVMKDSNGNFEIQRLIDDFLNISVLNYDNYHRI WFMRRKLGSWCKACVEFYGKPAKFQLTAHFENTMNLYEQALTEVLLGKTELLKFYDTLKG LYILLYWFTSEYSTFGNSIAFLDSSLGFTKFDFNFQRLIRIVLYVFDSCELAALEYAEIQ LKYISLVVDYVCNRTISTALDAPALVCCEQLKFVLTTMHHFLDNKYGLLDNDPTMAKGIL RLYSLCISNDFSKCFVDHFPIDQWADFSQSEHFPFTQLTNKALSIVYFDLKRRSLPVEAL KYDNKFNIWVYQSEPDSSLKNVTSPFDDRYKQLEKLRLLVLKKFNKTERGTLLKYRVNQL SPGFFQRAGNDFKLILNEASVSIQTCFKTNNITRLTSWTVILGRLACLESEKFSGTLPNS TKDMDNWYVCHLCDIEKTGNPFVRINPNRPEAAGKSEIFRILHSNFLSHPNIDEFSESLL SGILFSLHRIFSHFQPPKLTDGNGQINKSFKLVQKCFMNSNRYLRLLSTRIIPLFNISDS HNSEDEHTATLIKFLQSQKLPVVKENLVIAWTQLTLTTSNDVFDTLLLKLIDIFNSDDYS LRIMMTLQIKNMAKILKKTPYQLLSPILPVLLRQLGKNLVERKVGFQNLIELLGYSSKTI LDIFQRYIIPYAIIQYKSDVLSEIAKIMCDGDTSLINQMKVNLLKKNSRQIFAVALVKHG LFSLDILETLFLNRAPTFDKGYITAYLPDYKTLAEITKLYKNSVTKDASDSENANMILCS LRFLITNFEKDKRHGSKYKNINNWTDDQEQAFQKKLQDNILGIFQVFSSDIHDVEGRTTY YEKLRVINGISFLIIYAPKKSIISALAQISICLQTGLGLKEVRYEAFRCWHLLVRHLNDE ELSTVIDSLIAFILQKWSEFNGKLRNIVYSILDTLIKEKSDLILKLKPYTTLALVGKPEL GILARDGQFARMVNKIRSTTDLIPIFANNLKSSNKYVINQNLDDIEVYLRRKQTERSIDF TPKKVGQTSDITLVLGALLDTSHKFRNLDKDLCEKCAKCISMIGVLDVTKHEFKRTTYSE NEVYDLNDSVQTIKFLIWVINDILVPAFWQSENPSKQLFVALVIQESLKYCGLSSESWDM NHKELYPNEAKLWEKFNSVSKTTIYPLLSSLYLAQSWKEYVPLKYPSNNFKEGYKIWVKR FTLDLLKTGTTENHPLHVFSSLIREDDGSLSNFLLPYISLDIIIKAEKGTPYADILNGII IEFDSIFTCNLEGMNNLQVDSLRMCYESIFRVFEYCKKWATEFKQNYSKLHGTFIIKDTK TTNMLLRIDEFLRTTPSDLLAQRSLETDSFERSALYLEQCYRQNPHDKNQNGQLLKNLQI TYEEIGDIDSLDGVLRTFATGNLVSKIEELQYSENWKLAQDCFNVLGKFSDDPKTTTRML KSMYDHQLYSQIISNSSFHSSDGKISLSPDVKEWYSIGLEAANLEGNVQTLKNWVEQIES LRNIDDREVLLQYNIAKALIAISNEDPLRTQKYIHNSFRLIGTNFITSSKETTLLKKQNL LMKLHSLYDLSFLSSAKDKFEYKSNTTILDYRMERIGADFVPNHYILSMRKSFDQLKMNE QADADLGKTFFTLAQLARNNARLDIASESLMHCLERRLPQAELEFAEILWKQGENDRALK IVQEIHEKYQENSSVNARDRAAVLLKFTEWLDLSNNSASEQIIKQYQDIFQIDSKWDKPY YSIGLYYSRLLERKKAEGYITNGRFEYRAISYFLLAFEKNTAKVRENLPKVITFWLDIAA ASISEAPGNRKEMLSKATEDICSHVEEALQHCPTYIWYFVLTQLLSRLLHSHQSSAQIIM HILLSLAVEYPSHILWYITALVNSNSSKRVLRGKHILEKYRQHSQNPHDLVSSALDLTKA LTRVCLQDVKSITSRSGKSLEKDFKFDMNVAPSAMVVPVRKNLDIISPLESNSMRGYQPF RPVVSIIRFGSSYKVFSSLKKPKQLNIIGSDGNIYGIMCKKEDVRQDNQYMQFATTMDFL LSKDIASRKRSLGINIYSVLSLREDCGILEMVPNVVTLRSILSTKYESLKIKYSLKSLHD RWQHTAVDGKLEFYMEQVDKFPPILYQWFLENFPDPINWFNARNTYARSYAVMAMVGHIL GLGDRHCENILLDIQTGKVLHVDFDCLFEKGKRLPVPEIVPFRLTPNLLDALGIIGTEGT FKKSSEVTLALMRKNEVALMNVIETIMYDRNMDHSIQKALKVLRNKIRGIDPQDGLVLSV AGQTETLIQEATSEDNLSKMYIGWLPFW*
Coding Sequence Details
Insert Sequence Verified?:
N
Verification Method:
Insert was fully sequenced in parent vector
Reference Sequence Annotations:
Start on reference sequence
1
End on reference sequence
7107
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
yeast
Marker:
LEU2
Host Type: bacterial Marker: ampicillin
Host Type: bacterial Marker: ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
ZM552
Synonyms:
ZM552
Description:
Yeast expression vector with N-terminal 3X V5 epitope tag; ampicillin resistance in bacteria; LEU2 resistance in yeast; Gateway cloning.
Comments:
ZM511 Stu1 vector plus Gateway attR frame B cassette EcoRV fragment; same as ZM515. Cloning into the vector removes the ccdB cassette and chloramphenicol resistance.
Size (bp):
8302
Parent Vector:
None
Properties:
Gateway, acceptor (destination), recombinational cloning, with tag/fusion/marker, yeast expression
Author Name:
Zarmik Moqtaderi
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
primer site
M13F
M13 forward sequencing primer
8286
8301
primer site
M13R
M13 reverse sequencing primer
2630
2647
promoter
CMD1
CMD1 promoter
295
484
recombination site
attR1
attR1 recombinational cloning site
650
666
recombination site
attR2
attR2 recombination site
2337
2352
reporter gene
LacZ
LacZ alpha gene
8146
8214
selectable marker
AmpR
ampcillin resistance
3795
4454
selectable marker
CmR
chloramphenicol resistance. removed with the ccdB cassette in clones with insert
883
1539
selectable marker
LEU2
LEU2 selectable marker in yeast
5273
5344
tag
V5
N-terminal triple V5 tag
494
631
trxn termination sequence
CYC1
CYC1 terminator
2377
2582
yeast ARS
ARS
ARS yeast origin of replication
0
0
yeast centromere
CEN
yeast centromere
0
0
