Plasmid Collections

The 90/90 Human ORFeome V 3 - Transcription Factors Subcollection contains 2,064 unique ORFs from the 90/90 Human ORFeome V 3 that were identified as transcription factors, including 61 also in the Membrane Secreted Protein subcollection.

The 90/90 Human ORFeome V 3 contains over 17,000 unique ORFs from the human genome. This collection comprises the most comprehensive and complete version of the Human ORFeome to date. The goal of this collection is to eventually have one representative clone for gene clusters with at least 90% sequence coverage and 90% identity covering all ORFs in the Human genome. The Entry vector version of this collection enables the use of Gateway technology to quickly clone inserts into expression vectors for many experiments using the Gateway LR reaction.

Currently, a complete physical collection of cloned cDNAs encoding all human genes clusters does not exist. The 90/90 Human ORFeome Collection is a collection of full length ORFs containing one representative of each gene with cDNA length under 7,000 bases (and thus suitable for Gateway LR transfer to destination vectors), targeting the longest isoforms with evidence of existence and grouping gene families into clusters with a minimum of 90% protein sequence homology. That is, we target one representative clone for gene clusters with at least 90% sequence coverage and 90% identity, for a total of 18,216 gene clusters.

Based on the Human Genome RefSeq v73 (Nov 2015), there are 20,398 protein-coding human genes predicted to be in existence. Of these, 19,279 of these have isoforms with experimental evidence of expression. 19,029 genes are < 7kb in size, making them suitable for use in Gateway LR transfers to destination vectors for downstream expression experiments. There are 18,216 gene clusters with 90% sequence identity that cover > 90% of the gene lengths. From these gene clusters, we choose the longest isoform to be the representative clone.

Subcollections containing only ORFs denoted as Membrane Proteins and "Other" are also available.