Vector Name:
pLP-DS3xMyc
Synonyms:
pLP-DS-3xMyc
Sequencing Primer:
Forward:
M13F
Reverse:
T7 terminator
Description:
Vector for in vitro translation with mammalian extracts, with CITE region, adds 3xMyc tag; amp resistance; recombinational cloning.
Comments:
None
Size (bp):
3743
Parent Vector:
None
Properties:
Creator, acceptor (destination), in vitro transcription, loxP, ssDNA production, with tag/fusion/marker
Author Name:
Daniel Schiwek
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 origin of replication
1028
1710
primer site
M13 forward
M13 Forward primer binding site
3703
3720
recombination site
LoxP
LoxP site for recombinational cloning
618
651
selectable marker
AmpR
ampicillin resistance
1808
2467
ssDNA origin
f1
f1 origin for ssDNA production
3114
3554
tag
c-myc
Myc epitope tag open reading frame
522
611
trxn termination sequence
T7 term
T7 terminator sequence
800
846
