Vector Name:
pET23b
Synonyms:
pET-23b, pET23
Sequencing Primer:
Forward:
T7
Reverse:
T7 terminator
Description:
Bacterial expression vector with T7 promoter, adds N-terminal T7 tag and C-terminal His tag; amp resistance; restriction enzyme cloning.
Comments:
None
Size (bp):
3666
Parent Vector:
None
Properties:
bacterial expression, in vitro transcription, multiple cloning site, with tag/fusion/marker
Author Name:
Midwest Center for Structural Genomics
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (XhoI, NotI, EagI, HindIII, SalI, HincII, SacI, EcoRI, BamHI)
159
203
bacterial origin
ori
bacterial origin of replication
1430
2112
promoter
T7
T7 promoter
303
319
selectable marker
AmpR
ampicillin resistance gene
2211
3068
ssDNA origin
f1 ori
f1 origin
3200
3655
tag
T7 tag
T7 tag cds
207
239
tag
His
C-terminal 6xHis tag
140
157
trxn termination sequence
T7 term
T7 terminator sequence
26
72
