Vector Name:
pET28-N-His-SUMO
Synonyms:
N-His-SUMO pET28
Sequencing Primer:
Forward:
T7
Reverse:
ISFI_newrev
Description:
Expression vector with T7 promoter, adds cleavable N-terminal 6xHis tag, SUMO tag; kanamycin resistance; restriction enzyme cloning.
Comments:
Feature annotations are approximate based on the pET28a vector
Size (bp):
5369
Parent Vector:
None
Properties:
cell-free expression, in vitro transcription, multiple cloning site, with tag/fusion/marker
Author Name:
Integrated Center for Structure and Function Innovation
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Not Available
Vector Features:
Type
Name
Description
Start
End
promoter
T7
T7 promoter
367
386
tag
His
cleavable N-terminal His tag (inserted after the NcoI site in pET28a)
270
287
tag
SUMO
cleavable N-terminal His/SUMO tag
288
668
