Detailed Vector Information: pFASTBAC1

Vector Name:
pFASTBAC1
Synonyms:
pFB-1, pFB1
Sequencing Primer:
Forward:  pFB Forward
Reverse:  pFB Reverse
Description:
Transfer vector (to E. coli from expression bacmid) with polyhedrin promoter, adds SV40 polyA site; amp resistance (gentamycin for selection in insect cells); restriction enzyme cloning.
Comments:
requires transformation into E. coli DH10Bac for bacmid production
Size (bp):
4775
Parent Vector:
None
Properties:
baculovirus/insect cell expression, multiple cloning site
Author Name:
Midwest Center for Structural Genomics
Publications:
None
Vector Map:
Vector Sequence:
Sequence:

Vector Features:

Type
Name
Description
Start
End
MCS
MCS
MCS (includes BamHI, EcoRI, NotI, XhoI)
4037
4142
bacterial origin
pUC ori
pUC-type origin of replication
1594
2267
poly-A signal
polyA
SV40 polyA signal sequence
4205
4396
promoter
PH
polyhedrin promoter for insect cell expression
3904
4032
selectable marker
GenR
gentamycin resistance gene
3000
3533
selectable marker
AmpR
ampicillin resistance gene
787
1446
ssDNA origin
f1
f1 origin of replication
2
19
transposon fragment
Tn7L
Tn7L transposon site
4429
4594
transposon fragment
Tn7R
Tn7R transposon site
2709
2933