Vector Name:
pFASTBAC1-C-His-TOPO
Synonyms:
pFB1-C-His-TOPO, pFB1-C-His-TOPO_KW
Sequencing Primer:
Forward:
pFB forward
Reverse:
pFB reverse
Description:
Baculovirus vector, adds C-terminal 6xHis tag and TEV protease site; amp resistance; TOPO cloning.
Comments:
None
Size (bp):
4763
Parent Vector:
None
Properties:
TOPO Cloning, baculovirus/insect cell expression, with tag/fusion/marker
Author Name:
New York SGX Research Center for Structural Genomics
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
ATG start
start
initiation ATG (Met, Ala, Leu)
4065
4078
TOPO recognition site
TOPO
TOPO cloning site
4069
4078
bacterial origin
ori
pUC-type bacterial origin of replication
1544
2226
poly-A signal
polyA
SV40 polyA signal sequence
4189
4380
primer
pFB foward
forward sequencing primer: TATTCCGGATTATTCATACC
3995
4014
primer
pFB reverse
reverse sequencing primer: ACAAATGTGGTATGGCTGATT
4150
4170
promoter
PH
polyhedrin promoter for insect cell expression
3904
4032
selectable marker
GenR
gentamycin resistance gene
3000
3533
selectable marker
AmpR
ampicillin resistance gene
787
1446
ssDNA origin
f1 ori
f1 origin
19
325
tag
His
C-terminal 6xHis and stop
4079
4096
transposon fragment
Tn7L
Tn7L transposon site
1466
12
transposon fragment
Tn7R
Tn7R transposon site
2709
2933
