Vector Name:
pMCSG11
Synonyms:
pMCSG-11
Sequencing Primer:
Forward:
DuetUp1
Reverse:
T7 terminator
Description:
Coexpression vector with T7 promoter, adds N-terminal His tag, TEV, SNA; chloramphenicol resistance; ligation independent cloning (LIC).
Comments:
pACYCDuet-1 derivative, 2nd region replaced with LIC region
Size (bp):
4079
Parent Vector:
None
Properties:
bacterial expression, in vitro transcription, ligation independent cloning (LIC), with tag/fusion/marker
Author Name:
Midwest Center for Structural Genomics
Publications:
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
LIC
ligation-independent cloning (LIC) region
353
384
MCS
MCS
MCS (NcoI, BamHI, EcoRI, PstI, HindIII)
1801
1880
MCS
MCS2
MCS (BamHI, EcoRI, HindIII, AvaI)
2171
2216
bacterial origin
ori
P15A-type bacterial origin of replication
1821
2733
primer site
Duet Down1
Duet DOWN1 primer site
189
208
primer site
Duet P1
ACYC Duet U P1 primer site
4020
4039
primer site
Duet P2
Duet P2 primter site
189
208
promoter
T7
T7 promoter
214
230
promoter
T7 2
T7 promoter
4063
4079
protease cleavage site
TEV
TEV protease cleavage site
345
365
repressor binding site
lacO1
lacO sequence region
5
25
repressor binding site
lacO2
lacO sequence region
235
230
repressor protein gene
lacI
lacI cds
2857
3939
ribosome binding site
RBS1
ribosome binding site
58
63
ribosome binding site
RBS2
ribosome binding site
286
291
selectable marker
CmR
chloramphenicol resistance gene (CAT)
800
1459
tag
His
N-terminal His tag
83
100
tag
S-tag
N-terminal S-tag
437
481
tag
His 2
6xHis tag
303
320
trxn termination sequence
T7 term
T7 termination sequence
533
580
