Vector Name:
pMCSG14
Synonyms:
pMCSG-14
Sequencing Primer:
Forward:
T7
Reverse:
T7 terminator
Description:
Coexpression vector with T7 promoter, adds N-terminal His tag, GST, TEV, SNA; chloramphenicol resistance; ligation independent cloning (LIC).
Comments:
pACYCDuet-1 derivative, 2nd region replaced with LIC region
Size (bp):
4754
Parent Vector:
None
Properties:
bacterial expression, in vitro transcription, ligation independent cloning (LIC), with tag/fusion/marker
Author Name:
Midwest Center for Structural Genomics
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
LIC
Ligation independent cloning (LIC) site
1028
1059
bacterial origin
ori
P15A-type bacterial origin of replication
2496
3408
gene fragment
CAT
CAT coding sequence
1475
2134
promoter
T7
T7 promoter
214
230
protease cleavage site
TEV
TEV cleavage site cds
1020
1040
repressor binding site
lac operator
LacO fragment
5
25
repressor protein gene
LacI
LacI coding sequence
3532
4614
ribosome binding site
RBS
Ribosome binding site
58
63
ribosome binding site
RBS2
Ribosome binding site
286
291
tag
His
6xHis tag
83
100
tag
His 2
6xHis tag
978
995
tag
S-tag
S-tag
1112
1156
tag
GST
Glutathione transferase (GST) tag
339
998
trxn termination sequence
T7 term
T7 terminator sequence
1208
1255
