Detailed Vector Information: pMCSG17

Vector Name:
pMCSG17
Synonyms:
pMCSG-17
Sequencing Primer:
Forward:  T7
Reverse:  T7 terminator
Description:
Coexpression vector with T7 promoter, adds N-terminal Stag, TEV, SNA; amp resistance; ligation independent cloning (LIC).
Comments:
pMCSG7 derivative
Size (bp):
5316
Parent Vector:
None
Properties:
bacterial expression, in vitro transcription, ligation independent cloning (LIC), with tag/fusion/marker
Author Name:
Midwest Center for Structural Genomics
Publications:
PMID: 17363272
Title: New vectors for co-expression of proteins: structure of Bacillus subtilis ScoAB obtained by high-throughput protocols
PMID: 18988021
Title: A family of LIC vectors for high-throughput cloning and purification of proteins.
Vector Map:
Vector Sequence:
Sequence:

Vector Features:

Type
Name
Description
Start
End
MCS
LIC
Ligation independent cloning (LIC) site
204
235
bacterial origin
ori
ColE1-type bacterial origin of replication
3291
3973
promoter
T7
T7 promoter
390
406
protease cleavage site
TEV
TEV site cds
223
243
repressor binding site
lac operator
LacO fragment
365
387
repressor protein gene
LacI
LacI coding sequence
920
1879
selectable marker
AmpR
ampicillin resistance gene
4068
4928
tag
His
C-terminal 6xHis tag
140
157
tag
S-tag
N-terminal S-tag
271
315
trxn termination sequence
T7 term
T7 terminator
26
72