Vector Name:
pMCSG18
Synonyms:
pMCSG-18
Sequencing Primer:
Forward:
T7
Reverse:
T7 terminator
Description:
Screening vector with T7 promoter, adds N-terminal His tag, TEV, LICs, GFP; amp resistance; ligation independent cloning (LIC).
Comments:
pMCSG7 derivative
Size (bp):
6009
Parent Vector:
None
Properties:
bacterial expression, in vitro transcription, ligation independent cloning (LIC), with tag/fusion/marker
Author Name:
Midwest Center for Structural Genomics
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
LIC
Ligation independent cloning (LIC) site
927
958
bacterial origin
ori
ColE1-type bacterial origin of replication
3984
4666
promoter
T7
T7 promoter
1083
1099
protease cleavage site
TEV
TEV cleavage site cds
946
966
repressor binding site
lac operator
LacO fragment
1058
1080
repressor protein gene
LacI
LacI coding sequence
1613
2572
selectable marker
AmpR
ampicillin resistance gene
4761
5621
tag
His 2
C-terminal 6xHis tag
140
157
tag
GFP
GFP tag
208
927
tag
His
N-terminal 6xHis tag
991
1008
trxn termination sequence
T7 term
T7 terminator sequence
26
72
