Vector Name:
pMCSG22
Synonyms:
pMCSG-22
Sequencing Primer:
Forward:
T7
Reverse:
T7 terminator
Description:
Coexpression vector with T7 promoter, adds N-terminal His, Sloop, TEV, SNA; spectinomycin resistance; ligation independent cloning (LIC).
Comments:
pCDFDuet-1 derivative, 2nd region replaced with LIC region
Size (bp):
3906
Parent Vector:
None
Properties:
bacterial expression, in vitro transcription, ligation independent cloning (LIC), with tag/fusion/marker
Author Name:
Midwest Center for Structural Genomics
Publications:
PMID:
18988021
Title: A family of LIC vectors for high-throughput cloning and purification of proteins.
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
LIC
Ligation independent cloning (LIC) site
407
438
bacterial origin
ori
CloDF-type bacterial origin of replication
1736
2474
promoter
T7
T7 promoter
3890
3906
promoter
T7
T7 promoter (2)
214
230
promoter
T7 2
T7 promoter
3890
3906
protease cleavage site
TEV
TEV cleavage site cds
399
419
repressor binding site
lac operator
LacO fragment
5
25
repressor binding site
lac operator 2
LacO fragment
235
255
repressor protein gene
LacI
LacI coding sequence
2684
3643
ribosome binding site
RBS
Ribosome binding site
58
63
ribosome binding site
RBS2
Ribosome binding site
286
291
ribosome binding site
RBS 3
Ribosome binding site
1602
1606
selectable marker
SpectR
Spectinomycin resistance gene (aadA)
805
1596
tag
His
6xHis tag
83
100
tag
His 2
6xHis tag
303
320
tag
S loop
S loop
339
392
trxn termination sequence
T7 term
T7 terminator sequence
587
634
