Detailed Vector Information: pMCSG9

Vector Name:
pMCSG9
Synonyms:
pMCSG-9
Sequencing Primer:
Forward:  T7
Reverse:  T7 terminator
Description:
Vector with tags for solubility of insert, with T7 promoter, adds N-terminal His tag, MBP, TEV, SNA; amp resistance; ligation independent cloning (LIC).
Comments:
pMCSG7 derivative
Size (bp):
6405
Parent Vector:
None
Properties:
bacterial expression, improves solubility of insert, in vitro transcription, ligation independent cloning (LIC), with tag/fusion/marker
Author Name:
Midwest Center for Structural Genomics
Publications:
PMID: 16497515
Title: An expression vector tailored for large-scale, high-throughput purification of recombinant proteins
Vector Map:
Vector Sequence:
Sequence:

Vector Features:

Type
Name
Description
Start
End
MCS
LIC
ligation-independent cloning (LIC) site
204
235
bacterial origin
ori
pBR322-type bacterial origin of replication
4399
4399
promoter
T7
T7 promoter
1479
1495
protease cleavage site
TEV
TEV protease cleavage site cds
223
243
repressor binding site
lac operator
lacO regulatory sequence
1454
1476
repressor protein gene
LacI
LacI cds
2009
2968
selectable marker
AmpR
ampicillin resistance
5157
6017
tag
His
6xHis tag
140
157
tag
His 2
N-terminal 6xHis tag
1387
1404
tag
MBP
maltose binding protein cds
265
1362
trxn termination sequence
T7 term
T7 terminator
26
72