Vector Name:
pMH1TEV
Synonyms:
pMH-1-TEV
Sequencing Primer:
Forward:
pBAD forward
Reverse:
alt_bad_rev
Description:
Bacterial expression vector, arabinose or T7 induced expression, adds N-terminal MGSDKIHHHHHHENLYFQG tag; amp resistance; PIPE cloning.
Comments:
None
Size (bp):
4069
Parent Vector:
None
Properties:
PIPE cloning, bacterial expression, in vitro transcription, inducible expression, with tag/fusion/marker
Author Name:
Joint Center for Structural Genomics
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ori
ColE1-type bacterial origin of replication
1908
2590
inducible trxn activator protein gene
araC
araC cds
3163
4035
promoter
araC
araC promoter
256
285
protease cleavage site
TEV
TEV protease cleavage site
382
402
selectable marker
AmpR
ampicillin resistance gene
1151
1810
tag
His
N-terminal 6xHis tag
364
381
trxn regulatory element
arabinose O1 operator
arabinose O1 operator
5
23
trxn regulatory element
arabinose O2 operator
arabinose O2 operator
161
182
trxn termination sequence
rrnB
rrnB T2 transcriptional terminator
813
839
