Detailed Vector Information: pMH4TEV
Description:
Bacterial expression vector, arabinose or T7 induced expression, adds N-terminal MGSDKIHHHHHHENLYFQG tag; amp resistance; PIPE cloning.
Properties:
PIPE cloning, bacterial expression, in vitro transcription, inducible expression, with tag/fusion/marker
Author Name:
Joint Center for Structural Genomics
Type
Name
Description
Start
End
bacterial origin
ori
ColE1-type bacterial origin of replication
3352
4034
inducible trxn activator protein gene
araC
araC cds
5484
4606
negative selection marker
ccdB
ccdB negative selection cds (may be fone in with-insert form)
1597
1806
promoter
T7
T7 promoter
290
310
protease cleavage site
TEV
TEV protease cleavage site
382
402
selectable marker
CmR
chloramphenicol resistance gene (CmR)
505
1161
selectable marker
AmpR
ampicillin resistance gene
2595
3254
tag
His
N-terminal 6xHis tag
364
381
trxn regulatory element
arabinose O1 operator
arabinose O1 operator
161
182
trxn regulatory element
arabinose O2 operator
arabinose O2 operator
5
23
trxn termination sequence
rrnB
rrnB T2 transcriptional terminator
2259
2285