Detailed Vector Information: pSGX-TOPO

Vector Name:
pSGX-TOPO
Synonyms:
pSGX-TOPO_KF
Sequencing Primer:
Forward:  pFB forward
Reverse:  pFB reverse
Description:
Baculovirus vector, adds N-terminal 6xHis tag and TEV protease site; ampicillin resistance in bacteria (gentamicin resistance in insect cells); TOPO cloning.
Comments:
pFastBAC topogated N-His with TEV.
Size (bp):
4793
Parent Vector:
None
Properties:
TOPO Cloning, baculovirus/insect cell expression, with tag/fusion/marker
Author Name:
New York SGX Research Center for Structural Genomics
Publications:
None
Vector Map:
Vector Sequence:
Sequence:

Vector Features:

Type
Name
Description
Start
End
ATG start
start
initiation ATG
4050
4052
TOPO recognition site
TOPO
TOPO cloning site
4123
4132
bacterial origin
ori
pUC-type origin of replication
1544
2226
poly-A signal
polyA
SV40 poly-A signal sequence
4222
4413
primer
pFB foward
forward sequencing primer: TATTCCGGATTATTCATACC
3995
4014
primer
pFB reverse
reverse sequencing primer: ACAAATGTGGTATGGCTGATT
4183
4203
promoter
Pc
Pc promoter
0
0
promoter
PH
polyhedrin promoter
3904
4032
selectable marker
GenR
gentamycin resistance gene
3000
3533
selectable marker
AmpR
ampicillin resistance gene
787
1446
ssDNA origin
f1 ori
f1 origin
19
325
tag
His
6xHis tag
4062
4079
transposon fragment
Tn7L
Tn7L transposon sequence
0
0
transposon fragment
Tn7R
Tn7R transposon sequence
2709
2933