Vector Name:
pMCentr1
Synonyms:
pMCentr-1
Description:
cloning vector to link to Gateway system, with attL1-TEV-LIC site-attL2; zeocin resistance; ligation independent cloning to make pENTR vector for recombinational cloning
Comments:
From pDONRZeo; LIC generates ENTR vector
Size (bp):
2115
Parent Vector:
None
Properties:
Gateway, donor (entry), fluorescent marker, ligation independent cloning (LIC), mammalian expression, recombinational cloning, ssDNA production, with tag/fusion/marker
Author Name:
M.Donnelly
Midwest Center for Structural Genomics
PSI
Midwest Center for Structural Genomics
Midwest Center for Structural Genomics
PSI
Midwest Center for Structural Genomics
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ori
bacterial origin of replication
1439
2112
ligation independent cloning
LIC
ligation independent cloning (LIC)
679
710
primer site
M13 reverse
M13 reverse sequencing primer
851
867
primer site
M13 forward
sequencing primer site
537
552
protease cleavage site
TEV
protease cleavage site
671
691
recombination site
attL1
attL1 recombination site
569
668
recombination site
attL2
attL2 recombination site
711
810
selectable marker
ZeoR
zeocin resistance gene
935
1309
trxn termination sequence
rrnB T2
rrnB T2 transcription termination sequence
268
295
trxn termination sequence
rrnB T1
rrnB T1 transcription termination sequence
427
470
