Vector Name:
pMCentr3
Synonyms:
pMCentr-3
Description:
destination vector to accept inserts from pENTR clones; attL1-TVMV-LIC site-attL2; kanamycin resistance; ligation independent cloning to Gateway
Comments:
From pMCentr2; TVMV site instead of TEV site
Size (bp):
2586
Parent Vector:
None
Properties:
Gateway, acceptor (destination), fluorescent marker, ligation independent cloning (LIC), mammalian expression, recombinational cloning, ssDNA production, with tag/fusion/marker
Author Name:
M.Donnelly
Midwest Center for Structural Genomics
PSI
Midwest Center for Structural Genomics
Midwest Center for Structural Genomics
PSI
Midwest Center for Structural Genomics
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ori
bacterial origin of replication
0
0
ligation independent cloning
LIC
ligation independent cloning (LIC)
679
710
primer site
M13 reverse
M13 Reverse priming site
851
867
primer site
M13 forward
sequencing primer site
537
552
protease cleavage site
TVMV
protease cleavage site
671
691
recombination site
attL1
attL1 recombinational cloning site
569
668
recombination site
attL2
attL2 recombinational cloning site
711
810
selectable marker
KanR
kanamycin resistance gene
980
1789
trxn termination sequence
rrnB T2
rrnB T2 transcription termination sequence
268
295
trxn termination sequence
rrnB T1
rrnB T1 transcription termination sequence
427
470
