Vector Name:
pLIC-CH
Synonyms:
''
Sequencing Primer:
Forward:
T7
Reverse:
T7 terminator
Description:
Bacterial expression vector with C-terminal 8x His tag separated from the insert by a TEV protease cleavage site; kanamycin resistance in bacteria; ligation independent cloning (LIC)
Comments:
None
Size (bp):
5361
Parent Vector:
None
Properties:
bacterial expression
Author Name:
University of California San Francisco
Joy Chen
Center for Structures of Membrane Proteins
Joy Chen
Center for Structures of Membrane Proteins
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
ligation independent cloning
LIC
Ligation independent cloning site
5088
5095
phage origin
f1
f1 phage origin
29
335
promoter
T7
T7 promoter
4984
5003
protease cleavage site
TEV
TEV protease cleavage site; separates 8xHis from insert
5113
5133
repressor protein gene
LacI
LacI gene
3515
4531
selectable marker
KanR
Kanamycin resistance gene
563
1375
tag
His
C-terminal 8xHis tag
5134
5157
trxn regulatory element
lac operator
lac operator sequence
5003
5025
trxn termination sequence
T7 term
T7 transcription termination site
5292
5338
