Vector Name:
PCDF Bravo
Synonyms:
''
Sequencing Primer:
Forward:
T7
Reverse:
T7 terminator
Description:
Bacterial expression vector with T7 promoter, N-terminal His tag and C-terminal Flag tag; ligation independent cloning (LIC) clone; streptomycin resistance in bacteria.
Comments:
None
Size (bp):
3636
Parent Vector:
None
Properties:
bacterial expression, ligation independent cloning (LIC), with tag/fusion/marker
Author Name:
William Harries
Center for Structures of Membrane Proteins
PSI
Center for Structures of Membrane Proteins
PSI
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial operon
lac operator
lacO
3
25
bacterial origin
CloDF13
CloDF13 origin
1466
2204
gene fragment
LacI
LacI transcriptional represso
2417
3373
promoter
T7
T7 Promoter
3621
3636
ribosome binding site
RBS
RBS-1: ribosome binding site
58
63
selectable marker
StrepR
streptomycin resistance gene
535
1326
tag
His
N-terminal 10xHis tag
170
199
tag
Flag
C-terminal Flag tag
80
103
trxn termination sequence
T7 term
T7 terminator
306
324
