Vector Name:
p4-Ib
Synonyms:
''
Sequencing Primer:
Forward:
p4
Reverse:
Unknown
Description:
Mycoplasma coexpression expression vector with the Pimyc promoter and C-termial c-myc tag; hygromycin resistance in bacteria and mycobacteria; restriction enzyme cloning (AsiSI, PmeI).
Comments:
Target gene controlled by Pimyc promoter for expression is cloned between AsiSI and PmeI restriction sites.
Size (bp):
4928
Parent Vector:
None
Properties:
multiple cloning site, with tag/fusion/marker
Author Name:
Brian Fox
Yong Chang
David Mead
Vinay Dhodda
Phil Brumm
Center for Eukaryotic Structural Genomics
PSI
Yong Chang
David Mead
Vinay Dhodda
Phil Brumm
Center for Eukaryotic Structural Genomics
PSI
Publications:
PMID:
19760663
Title: One-plasmid tunable coexpression for mycobacterial protein-protein interaction studies
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
multiple cloning site
220
334
bacterial origin
ColE
ColE1 replication origin (on complementary strand)
1013
1547
mycobacterial origin
myc ori
mycobacterial origin of replication
1734
3579
promoter
Pimyc
Pimyc promoter
84
219
selectable marker
HygR
hygromycin resistance cassette
3916
4914
tag
His
His tag
271
303
tag
myc
C-terminal c-myc tag
271
306
trxn termination sequence
rrnB
rrnB T2 transcriptional terminator
49
69
trxn termination sequence
T4g32
T4g32 transcription terminator
349
369
