Detailed Vector Information: p9T-H

Vector Name:
p9T-H
Synonyms:
''
Sequencing Primer:
Forward:  Phsp
Reverse:  Unknown
Description:
Mycoplasma coexpression expression vector with the PtetO promoter and Phsp promoter; tetracycline-inducible expression of insert; hygromycin resistance in bacteria and mycobacteria; restriction enzyme cloning (PtetO gene: AsiSI, PmeI; Phsp gene: PacI, SbfI).
Comments:
Target gene controlled by PtetO promoter for expression is cloned between AsiSI and PmeI restriction sites. Then, the second gene controlled by Phsp promoter for expression is cloned between PacI and SbfI restriction sites.
Size (bp):
8203
Parent Vector:
None
Properties:
multiple cloning site
Author Name:
Brian Fox
Yong Chang
David Mead
Vinay Dhodda
Phil Brumm
Center for Eukaryotic Structural Genomics
PSI
Publications:
PMID: 19760663
Title: One-plasmid tunable coexpression for mycobacterial protein-protein interaction studies
Vector Map:
Vector Sequence:
Sequence:

Vector Features:

Type
Name
Description
Start
End
MCS
MCS
Multiple cloning site
376
412
bacterial origin
ColE
ColE1 replication origin (on complementary strand)
6803
7336
mycobacterial origin
myc ori
mycobacterial origin of replication
1490
3964
promoter
PtetO
UV15 with two tet operators; tetracycline-inducible promoter
72
370
promoter
Psmyc
Psmyc promoter
4316
4632
promoter
Phsp
Phsp promoter
962
1333
repressor binding site
TET
tetR(B) Tet Repressor class B
4659
5282
selectable marker
HygR
hygromycin resistance cassette (on complementary strand)
5389
6387
trxn termination sequence
rrnB
3 rrnBT2 transcriptional terminators; located from nucleotides 24-43, 913-932 and 4276-4295
24
43
trxn termination sequence
T4g32
2 T4g32 transcription terminator; located from nucleotides 1381-1399 and 5318-5336
1381
1399