Mycoplasma coexpression expression vector with the PtetO promoter and Pimyc promoter; tetracycline-inducible expression of insert; hygromycin resistance in bacteria and mycobacteria; restriction enzyme cloning (PtetO gene: AsiSI, PmeI; Pimyc gene: PacI, SbfI).
Comments:
Target gene controlled by PtetO promoter for expression is cloned between AsiSI and PmeI restriction sites. Then, the second gene controlled by Pimyc promoter for expression is cloned between PacI and SbfI restriction sites.
Size (bp):
8203
Parent Vector:
None
Properties:
multiple cloning site
Author Name:
Brian Fox
Yong Chang
David Mead
Vinay Dhodda
Phil Brumm
Center for Eukaryotic Structural Genomics
PSI