Vector Name:
pCPD_nGST-SBP_DC
Synonyms:
pCPD9-GST, pCPD_GST
Sequencing Primer:
Forward:
pCPD5698_F
Reverse:
pCPD6268_R
Description:
Bacterial expression vector with a T7 promoter, N-terminal GST, Thrombin prtease cleavage site, SBP tag, and TEV protease cleavage site; ampicillin and chloamphenicol resistance in bacteria (CmR removed in clones with inserts); Gateway recombinational cloning.
Comments:
One in a series of pCPD vectors made by J. Saul for E. coli protein expression at CPD.
Size (bp):
7710
Parent Vector:
None
Properties:
Gateway, acceptor (destination), bacterial expression, recombinational cloning, with tag/fusion/marker
Author Name:
Center for Personalized Diagnostics
Joshua LaBaer
Ji Qiu
Justin Saul
Joshua LaBaer
Ji Qiu
Justin Saul
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 replication origin
1344
2026
death cassette
ccdB
death cassette (removed when insert is present)
5864
7518
death cassette 2
ccdB (BsrGI removed)
ccdB death cassette with BsrGI restriction enzyme cleavage site removed (death cassette removed when insert is present)
7073
7378
primer
pCPD6268_R
reverse sequencing primer agccaactcagcttccttt
7598
7616
primer
pCPD5698_F
forward sequencing primer aattcatggacgagaagacc
5693
5712
promoter
T7
T7 promoter
4911
4927
protease cleavage site
Thrombin
thrombin cleavage site
5674
5691
protease cleavage site
TEV
TEV protease cleavage site
5818
5838
repressor protein gene
LacI
LacI repressor
3438
4397
ribosome binding site
RBS
pET ribosome binding site
4984
4990
selectable marker
AmpR
ampicillin resistance gene
587
1246
selectable marker
CmR
chloramphenicol resistance (removed when insert is present)
6072
6728
tag
GST
GST tag
4999
5667
tag
SBP
Streptavidin Binding Peptide tag
5698
5811
trxn regulatory element
lac operator
lac operator sequence
4930
4952
trxn termination sequence
T7 term
T7 terminator
7368
7685
