Vector Name:
pCPD_nMBP-SBP_DC
Synonyms:
pCPD9-MBP
Sequencing Primer:
Forward:
pCPD5698_F
Reverse:
pCPD6268_R
Description:
with tag/fusion/marker assay, Gateway and reg, acceptor (destination), recombinational cloning clone, bacterial expression expression with pCPD5698_F and pCPD6268_R and MBP and Thrombin and SBP and TEV and ccdB and CmR and T7 term and LacI and AmpR and ColE and T7 and lac operator and RBS; chloramphenicol resistance in bacterial, ampicillin resistance in bacterial;
Comments:
One in a series of pCPD vectors made by J. Saul for E. coli protein expression at CPD.
Size (bp):
8193
Parent Vector:
None
Properties:
Gateway, acceptor (destination), bacterial expression, recombinational cloning, with tag/fusion/marker
Author Name:
Center for Personalized Diagnostics
Joshua LaBaer
Ji Qiu
Justin Saul
Joshua LaBaer
Ji Qiu
Justin Saul
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 replication origin
1344
2026
death cassette
ccdB
ccdB death cassette (removed in clones with insert)
6347
8001
primer
pCPD5698_F
forward sequencing primer aattcatggacgagaagacc
6176
6195
primer
pCPD6268_R
reverse sequencing primer agccaactcagcttccttt
8079
8099
promoter
T7
T7 promoter
4911
4927
protease cleavage site
Thrombin
thrombin cleavage site
6157
6174
protease cleavage site
TEV
TEV protease cleavage site
6301
6321
repressor protein gene
LacI
LacI repressor
3438
4397
ribosome binding site
RBS
pET ribosome binding site
4984
4990
selectable marker
AmpR
Ampicillin resistance gene
587
1246
selectable marker
CmR
chloramphenicol resistance gene
6555
7211
tag
MBP
maltose binding protein tag
4999
6150
tag
SBP
Streptavidin Binding Peptide tag
6181
6294
trxn regulatory element
lac operator
LacO sequence
4930
4952
trxn termination sequence
T7 term
T7 terminator
8121
8168
