Vector Name:
pJFT7_cHalo_empty
Synonyms:
''
Description:
In vitro expression vector with T7 promoter and C-terminal TEV-cleavable Halo tag; does not contain a death cassette; ampicillin resistance; restriction enzyme cloning
Comments:
Modified from pANT7_cGST, expresses Halo without an insert
Size (bp):
4520
Parent Vector:
None
Properties:
cell-free expression, multiple cloning site, with tag/fusion/marker
Author Name:
Joshua LaBaer
Justin Saul
Fernanda Festa
Center for Personalized Diagnostics
Justin Saul
Fernanda Festa
Center for Personalized Diagnostics
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 origin
1805
2487
bacterial origin
M13 Ori
M13 origin
3881
4336
enhancer
CITE
cap-independent translational enhancer (CITE)
17
515
primer
HaloseqF
HaloseqF forward sequencing primer aagcctgcctaactgcaa
1373
1390
primer
cHaloseqR
cHalo reverse sequencing primer CAACATCGACGTAGTGCAT
666
684
primer
M13 forward
M13 forward sequencing primer
4480
4497
promoter
T7
T7 promoter
4496
4520
protease cleavage site
TEV
TEV protease cleavage site (EDLYFQS)
573
593
reporter gene
LacZ
LacZ alpha gene
4341
4409
selectable marker
AmpR
ampicillin resistance gene
2585
3244
tag
HALO
C-terminal Halo tag
609
1493
trxn termination sequence
T7 term
T7 terminator
1565
1583
