Vector Name:
pcDNA3.1/myc-His(-)A
Synonyms:
''
Sequencing Primer:
Forward:
Unknown
Reverse:
Unknown
Description:
Mammalian expression vector with C-terminal myc and 6xHis tags; ampicillin resistance in bacteria; restriction enzyme cloning.
Comments:
Sundquist Lab internal database number = WISP 02-257; Restriction enzyme cloning can be used with this vector - e.g. Xho1, BamHI works well
Size (bp):
5522
Parent Vector:
None
Properties:
mammalian expression, multiple cloning site, with tag/fusion/marker
Author Name:
University of Utah
Wesley I. Sundquist
Wesley I. Sundquist
Publications:
PMID:
19525971
Title: Structural basis for ESCRT-III protein autoinhibition.
Vector Map:
Vector Sequence:
Sequence:
Not Available
Vector Features:
Type
Name
Description
Start
End
bacterial operon
ColE
ColE1 bacterial origin
3749
4431
primer
forward primer
pcDNAF forward sequencing primer AGAGAACCCACTGCTTACTGGCTTATC
831
857
primer
reverse primer
pcDNAR reverse sequencing primer AACTAGAAGGCACAGTCGAGGCTG
1110
1133
promoter
T7
T7 promoter
863
882
selectable marker
AmpR
Ampicillin resistance
4529
5188
ssDNA origin
f1
f1 origin
1406
1712
tag
c-myc
C-terminal c-myc tag
1008
1036
tag
His
C-terminal 6x His tag
1052
1069
trxn regulatory element
lac operator
lac operator
3387
3409
