Detailed Vector Information: pJSP6_nHalo_empty

Vector Name:
pJSP6_nHalo_empty
Synonyms:
pEU-nHalo_empty
Sequencing Primer:
Forward:  HaloseqF
Reverse:  pF1KseqR
Description:
Wheat germ expression vector with SP6 promoter and TEV-cleavable N-terminal HALO tag; ampicillin resistance in bacteria; gateway cloning
Comments:
Modified from pEU_HSBC, expresses Halo tag without an insert
Size (bp):
4775
Parent Vector:
None
Properties:
Gateway, acceptor (destination), cell-free expression, recombinational cloning, with tag/fusion/marker
Author Name:
Arizona State University
Joshua LaBaer
Ji Qiu
Justin Saul
Center for Personalized Diagnostics
Publications:
None
Vector Map:
Vector Sequence:
Sequence:

Vector Features:

Type
Name
Description
Start
End
IRES
TMV Omega
TMV Omega IRES
9
131
bacterial origin
ColE
ColE1 bacterial origin
2229
2911
primer
reverse primer
pF1KseqR reverse sequencing primer CTTTCGGGCTTTGTTAGCAG
1163
1182
primer
forward primer
HaloseqF forward sequencing primer aagcctgcctaactgcaa
902
919
promoter
SP6
SP6 promoter
4759
4775
protease cleavage site
TEV
TEV protease cleavage site EDLYFQS
1035
1055
selectable marker
AmpR
Ampicillin resistance
3009
3668
tag
HALO
N-terminal HALO tag
132
1022
trxn termination sequence
T7 term
T7 terminator
1132
1145