Wheat germ expression vector with a SP6 promoter and N-terminal TEV-cleavable HALO tag; ampicillin resistance in bacteria and chloramphenicol resistance in empty vector; Gateway or Flexi cloning
Comments:
Modified from pEU_HSBC, can be used for either Gateway or Flexi cloning, contains Gateway Death Cassette
Size (bp):
6460
Parent Vector:
None
Properties:
Gateway, acceptor (destination), cell-free expression, recombinational cloning, with tag/fusion/marker
Author Name:
Arizona State University
Joshua LaBaer
Ji Qiu
Justin Saul
Center for Personalized Diagnostics
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
IRES
TMV Omega
TMV Omega IRES
9
131
bacterial origin
ColE
ColE1 bacterial origin
3914
4596
death cassette
ccdB
Gateway ccdB death cassette - not present in plasmids containing a gene insert
1096
2750
primer
forward primer
HaloseqF forward sequencing primer aagcctgcctaactgcaa
902
919
primer
reverse primer
pFK1seqR reverse sequencing primer CTTTCGGGCTTTGTTAGCAG