Detailed Vector Information: pCMV(delta2)

Vector Name:
pCMV(delta2)
Synonyms:
WISP12-93
Sequencing Primer:
Forward:  pcDNAF
Reverse:  pcDNAR
Description:
Mammalian expression vector with a deletion mutant of the CMV promoter; ampicillin resistance in bacteria, neomycin resistance in mammalian cells; restriction enzyme cloning
Comments:
Empty vector Sundquist Lab internal database number = WISP12-93. Restriction enzyme cloning can be used with this vector - e.g. Xho1, Kpn1 works well
Size (bp):
5061
Parent Vector:
Name: pCMV(WT)
Description: Vector containing the full length CMV promoter
Properties:
mammalian expression, multiple cloning site
Author Name:
University of Utah
Wesley I. Sundquist
Eiji Morita
Jun Arii
Devin Christensen
Jorg Votteler
Publications:
PMID: 22877307
Title: Attenuated protein expression vectors for use in siRNA rescue experiments
Vector Map:
Vector Sequence:
Sequence:

Vector Features:

Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 origin
3288
3970
primer
forward
pcDNAF forward 5' sequencing primer AGAGAACCCACTGCTTACTGGCTTATC
649
672
primer
reverse primer
pcDNAR reverse 3' sequencing primer AACTAGAAGGCACAGTCGAGGCTG
414
440
promoter
CMV
Truncated CMV promoter
234
498
selectable marker
AmpR
Ampicillin resistance
4068
4727
selectable marker
NeoR
neomycin resistance (mammalian cells)
1769
2560
ssDNA origin
f1
f1 origin
945
1251