Vector Name:
pET151-D-Topo
Synonyms:
''
Sequencing Primer:
Forward:
T7
Reverse:
T7 terminator
Description:
Bacterial expression vector with a T7 promoter and a TEV cleavable N-terminal 6xHis and V5 tag; amipcillin resistance in bacteria; TOPO cloning
Comments:
None
Size (bp):
5760
Parent Vector:
None
Properties:
TOPO Cloning, bacterial expression
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
TOPO recognition site
TOPO
TOPO cloning site
387
400
bacterial origin
pBR322 origin
pBR322 origin
1953
2626
promoter
T7
T7 promoter
209
225
protease cleavage site
TEV
TEV protease cleavage site
360
380
repressor protein gene
LacI
LacI gene
4500
5612
ribosome binding site
RBS
Ribosome binding site
282
289
selectable marker
AmpR
ampicillin resistance gene
948
1808
tag
His
N-terminal 6xHis tag
300
317
tag
V5
N-terminal V5 epitope tag
318
359
trxn regulatory element
lac operator
lac operator (lacO)
228
252
trxn termination sequence
T7 term
T7 termination
416
544
