Vector Name:
pJExpress414-His-PP
Synonyms:
''
Sequencing Primer:
Forward:
Unknown
Reverse:
Unknown
Description:
Bacterial expression vector with T7 promoter and 3C prescission protease-cleavable N-terminal 6xHis V5 tag; ampicillin resistance in bacteria; restriction enzyme cloning
Comments:
None
Size (bp):
4079
Parent Vector:
None
Properties:
bacterial expression, ligation independent cloning (LIC), with tag/fusion/marker
Author Name:
DNA2.0
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
promoter
T7
T7 promoter
1423
1442
protease cleavage site
3C
3C prescission protease cleavage site
1572
1595
repressor binding site
lac operator
lac operator
1442
1464
selectable marker
AmpR
Ampicillin resistance
2252
2911
tag
His
N-terminal 6xHis tag
1512
1529
tag
V5
N-terminal V5 tag
1530
1571
trxn termination sequence
T7 term
T7 terminator
1607
1653
