Vector Name:
pJFT7_nGST_DC
Synonyms:
''
Description:
In vitro expression vector with a T7 promoter and N-terminal TEV-cleavable GST tag; ampicillin resistance; Gateway or Flexi cloning compatible
Comments:
Vector has tendency to recombine between repeat sequences that flank the att sites resulting in decreased LR efficiency
Size (bp):
5998
Parent Vector:
None
Properties:
Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name:
Joshua LaBaer
Justin Saul
Ji Qiu
Fernanda Festa
Justin Saul
Ji Qiu
Fernanda Festa
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 bacterial origin
3283
3965
bacterial origin
M13 Ori
M13 origin
5359
5814
death cassette
ccdB
ccdB death cassette (removed in clones containing an insert)
2479
2784
enhancer
CITE
CITE enhancer
17
515
primer
forward primer
GSTseqF forward sequencing primer gcaagtatatagcatggcct
1105
1124
promoter
T7
T7 promoter
2982
5998
protease cleavage site
TEV
TEV protease cleavage site (EDLYFQS)
1209
1229
recombination site
attR1
attR1 recombinational cloning site
1252
1269
recombination site
attR2
attR2 recombinational cloning site
2925
2942
reporter gene
LacZ
LacZ alpha
5819
5887
selectable marker
CmR
Chloramphenicol resistance (removed in plasmids containing an insert)
1478
2134
selectable marker
AmpR
Ampicillin resistance marker
4063
4772
ssDNA origin
f1
f1 origin
5491
5797
tag
GST
N-terminal TEV-cleavable GST tag
525
1190
trxn termination sequence
T7 term
T7 terminator
3054
3101
