Bacterial co-expresion vector with T7 promoter and N-terminal TEV-cleavable 6xHis tag; contains argU and ileX rare tRNA; ampicillin resistance in bacteria; ligation independent cloning
Comments:
LIC regions plus a portion of the beta-lactamase gene from pMCSG60 removed by digestion with BglI and XbaI then ligated into pMCSG53 ligested with the same enzymes. Product is dual-LIC plasmid with truncated ori and the argU & ileX tRNA genes