Detailed Vector Information: pMCSG70

Vector Name:
pMCSG70
Synonyms:
''
Sequencing Primer:
Forward:  T7
Reverse:  T7 terminator
Description:
Bacterial expression vector with T7 promoter and C-terminal TEV-cleavable 10xHis tag and TVMV-cleavable MBP tag; contains argU and ileX rare tRNA; ampicillin resistance in bacteria; ligation independent cloning
Comments:
LIC region plus a portion of the beta-lactamase gene from pMCSG29 removed by digestion with BglI and XbaI then ligated into pMCSG53 digested with the same enzymes. Product is pMCSG29 with truncated ori and the argU & ileX tRNA genes
Size (bp):
5911
Parent Vector:
Name: pMCSG53
Description:
Properties:
bacterial expression, ligation independent cloning (LIC), with tag/fusion/marker
Author Name:
Midwest Center for Structural Genomics
PSI:Biology
Midwest Center for Structural Genomics
Publications:
PMID: 24057978
Title: New LIC vectors for production of proteins from genes containing rare codons
Vector Map:
Vector Sequence:
Sequence:

Vector Features:

Type
Name
Description
Start
End
3pUTR
2 micron ori
0
0
bacterial origin
pBR322 origin
pBR322 bacterial origin
3904
4492
ligation independent cloning
LIC
ligation independent cloning site
1382
1400
promoter
T7
T7 promoter
1463
1479
protease cleavage site
TEV
TEV protease cleavage site
1368
1388
protease cleavage site
TVMV
TVMV protease cleavage site
1311
1331
repressor binding site
lac operator
lac operator
1438
1460
repressor protein gene
LacI
LacI
2516
3475
selectable marker
AmpR
Ampicillin resistance
4663
5523
tRNA
ileX
ileX rare tRNA
2026
2213
tRNA
argU
argU rare tRNA
1546
1902
tag
His
C-terminal 10xHis tag
1332
1361
tag
MBP
C-terminal maltose binding protein tag
204
1310