Vector Name:
pMCSG77
Synonyms:
''
Sequencing Primer:
Forward:
T7
Reverse:
T7 terminator
Description:
Bacterial co-expression vector with T7 promoter and N-terminal TEV-cleavable 6xHis tag; contains argU and ileX rare tRNA; kanamycin resistance in bacteria; ligation independent cloning
Comments:
None
Size (bp):
5445
Parent Vector:
None
Properties:
bacterial expression, ligation independent cloning (LIC), with tag/fusion/marker
Author Name:
Midwest Center for Structural Genomics
PSI:Biology
Midwest Center for Structural Genomics
PSI:Biology
Midwest Center for Structural Genomics
Publications:
PMID:
24057978
Title: New LIC vectors for production of proteins from genes containing rare codons
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
p15A origin
p15A origin
4493
5322
ligation independent cloning
LIC
MCSG7 LIC site
260
291
ligation independent cloning
LIC2
MCSG26 LIC site
223
244
promoter
T7
T7 promoter
416
432
protease cleavage site
TEV
TEV protease cleavage site
279
299
repressor binding site
lac operator
lac operator
391
413
repressor protein gene
LacI
LacI
1326
2405
ribosome binding site
RBS
ribosome binding site
243
248
ribosome binding site
RBS2
ribosome binding site
353
358
selectable marker
KanR
kanamycin resistance
3350
4165
tRNA
ileX
ileX rare tRNA
1015
1166
tRNA
argU
argU rare tRNA
499
855
tag
His
N-terminal 6xHis tag
234
241
trxn termination sequence
T7 term
T7 terminator
26
72
