Vector Name:
pLIFE-miRNA
Synonyms:
''
Description:
Mammalian expression vector for expressing miRNAs; also contains red fluorescent protein, gateway cassette located within an intron of RFP 3'UTR, for ncRNA expression; ampicillin resistance in bacteria; Gateway cloning with L2R3 Gateway Cassette
Comments:
Growth before gateway recombination requires DB3.1 E. coli cells.
Size (bp):
6500
Parent Vector:
None
Properties:
Gateway, acceptor (destination), fluorescent marker, mammalian expression, recombinational cloning
Author Name:
Arizona State University
Marco Mangone
Justin Wolter
Center for Personalized Diagnostics
Marco Mangone
Justin Wolter
Center for Personalized Diagnostics
Publications:
PMID:
25074381
Title: 3pLIFE: a functional assay to detect miRNA targets in high-throughput
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
3pUTR
beta globin
rabbit beta-globin polyadenylation signal
2484
3035
bacterial origin
ColE
ColE1 origin
4143
4731
fluorescent marker
DsRed
mammalian codon-optimized tetrameric variant of DsRed fluorescent protein
7
684
primer
M13 forward
M13 forward sequencing primer
2412
2428
promoter
CMV
CMV promoter
5925
6432
selectable marker
CmR
Chloramphenicol resistance (part of theL2R3 Gateway Cassette); removed in plasmids containing a miRNA
1516
2172
selectable marker
AmpR
ampicillin resistance
4902
5867
splice acceptor site
3' splice acceptor
3'splice site
2435
2483
splice donor site
5' splice donor
5' splice site
693
701
