Vector Name:
pSFV4
Synonyms:
''
Sequencing Primer:
Forward:
pFAB007
Reverse:
Unknown
Description:
Bacterial expression vector for recombinant antibody expression with a Tac promoter and C-terminal Avi and His tag; ampicillin resistance in bacteria
Comments:
None
Size (bp):
7834
Parent Vector:
None
Properties:
bacterial expression, loxP, with tag/fusion/marker
Author Name:
Structural Genomics Consortium
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
pBR322 origin
pBR322 origin
1612
2231
gene fragment
light chain
pelB leader sequence and the anitbody light chain
4796
5128
gene fragment
heavy chain
Heavy chain
5625
6470
promoter
LacZ
LacZ a reporter
2950
3109
promoter
lac
Lac promoter
3171
3200
promoter
lac 2
Second lac promoter
4497
4520
promoter
tac promoter
Tac promoters
4607
4635
promoter
tac promoter 2
second Tac promoter
4702
4730
recombination site
LoxP
LoxP site
5143
5176
repressor protein gene
LacI
Lac I
3249
4340
selectable marker
AmpR
Ampicillin resistance
597
1457
ssDNA origin
f1
f1 origin
29
335
tag
AviTag
C-terminal Avi tag
6063
6473
tag
His
C-terminal 6xHis tag
7654
7671
trxn termination sequence
TrpA
TrpA terminator
6657
6684
